摘要
从河北省围场县某养殖场患病死亡的犊牛脏器样品中分离到1株优势菌株,对其进行常规形态学观察、生化鉴定、16S r DNA基因序列测定、动物致病性试验及药敏试验。结果显示,分离菌株的生化鉴定特性与粪肠球菌的生化鉴定特性相符;分离菌株16Sr DNA基因序列与粪肠球菌的同源性为96. 2%;在系统发育进化树上分离菌株与粪肠球菌聚为一簇;分离菌株对小白鼠具有一定的致病性,并将其命名为EFC QHD-1;分离菌株EFC QHD-1对替米考星、复方新诺明、卡那霉素等耐药;对环丙沙星、氟苯尼考、阿莫西林等敏感。
One dominant strain was isolated from a substantial organ sample of a diseased death calf in a farm in Weichang County,Hebei Province.It was subjected to routine morphological observation,biochemical identification,16S rDNA gene sequencing,animal pathogenicity test and drug susceptibility test.The results showed that the biochemical identification results of the isolate were consistent with Enterococcus faecalis ;the homology of the isolate with Enterococcus faecalis was 96.2% as determined by the 16S rDNA gene sequence;the isolates and Enterococcus faecalis clustered on the phylogenetic tree;the isolated strains had certain pathogenicity to mice,being named EFC QHD-1;the isolated strain EFC QHD-1 was resistant to compound sulfamethoxazole,tilmicosin and kanamycin,etc.and sensitive to ciprofloxacin,florfenicol and amoxicillin,etc.
作者
朱利霞
王洪彬
赵希艳
高桂生
史秋梅
高光平
ZHU Lixia;WANG Hongbin;ZHAO Xiyan;GAO Guisheng;SHI Qiumei;GAO Guangping(Hebei Key Laboratory of Preventive Veterinary Medicine,Hebei Normal University of Science & Technology,Qinhuangdao Hebei,066600,China)
出处
《河北科技师范学院学报》
CAS
2018年第4期36-40,共5页
Journal of Hebei Normal University of Science & Technology
基金
河北省二期农业产业技术体系-肉牛疫病防控岗位(项目编号:HBCT2018130203WSZ)
河北省高层次人才资助项目(项目编号:A2016005046)
关键词
犊牛
粪肠球菌
分离与鉴定
药敏试验
calf
Enterococcus faecalis
isolation and identification
drug susceptibility test
