摘要
以蓝果忍冬易落果品种‘海参崴’和不易落果品种‘蓓蕾’为试材,制作石蜡切片观察果柄离区的解剖结构,用高效液相色谱法测定果柄离区内源激素含量,并用酶联免疫吸附测定试剂盒测细胞壁相关酶活性,分析蓝果忍冬不同品种果柄离区形成过程中内源激素含量与细胞壁相关酶活性的变化特征,探讨二者与果实脱落的关系。结果表明:(1)‘蓓蕾’果柄离区在整个果实生长期均无明显断裂,‘海参崴’果柄离区在果实成熟期开始断裂,最终仅靠维管束连接。(2)海参崴’和‘蓓蕾’果柄离区激素水平和变化存在明显差异,在果实成熟期‘海参崴’的脱落酸(ABA)和乙烯(ETH)含量高于‘蓓蕾’,而生长素(IAA)和玉米素(ZT)含量低于‘蓓蕾’;在转色期赤霉素(GA3)含量低于‘蓓蕾’。(3)进入果实成熟期后‘海参崴’的纤维素酶(Cx)、β-葡萄糖苷酶(β-glu)活性升高,到转色期后期多聚半乳糖醛酸酶(PG)、果胶甲酯酶(PME)活性达到峰值;在整个果实生长期‘蓓蕾’的Cx活性基本没有变化,PG、PME、β-glu活性在生长早期即达到峰值,进入果实成熟期呈下降趋势。(4)‘海参崴’果柄离区Cx活性与IAA/ABA显著负相关(P<0.05);‘蓓蕾’果柄离区β-glu活性与IAA/ABA显著负相关(P<0.05),其PME活性与ABA含量呈极显著正相关关系(P<0.01)。研究认为,‘海参崴’果实易脱落主要与果实成熟期果柄离区ABA含量增加和IAA含量降低及细胞壁代谢酶Cx和PG活性变化有关,且Cx活性受IAA和ABA相对含量比值的负调节;‘蓓蕾’果柄离区PME活性增强也可以促进其果实脱落,PME活性受ABA含量的变化正向调节。
The cultivar ‘Vladivostok’ which was easy to drop and cultivar‘Berel’which was not easy to drop were both used as test materials.Paraffin section technique was used to observe the anatomical structure of the carpopodium abscission zone.We determined the contents of endogenous hormones in the carpopodium abscission zone by high performance liquid chromatography,and also determined the active of cell wall related enzymes by enzyme linked immunosorbent assay(ELISA)technique.we analyzed changes of endogenous hormones and cell wall-related enzyme activities in different Lonicera cearulea L.during the formation of the carpopodium abscission zone,and hope to reveal the relationship between endogenous hormones and cell wall-related enzymes and their effects on fruit abscission.The results showed:(1)there were no obvious fracture in ‘Berel’carpopodium abscission zone at the whole fruit growth period,but‘Vladivostok’carpopodium abscission zone was gradually disintegration when the fruit matured,ultimately.It relies on vascular bundle connected between fruit and carpopodium.(2)There was a significant difference in hormone levels and trends in carpopodium abscission zone between ‘Berel’and ‘Vladivostok’.At maturity,the ABA and ETH contents of‘Vladivostok’were higher than that of‘Berel’,while the IAA and ZT contents of‘Vladivostok’were lower than that of‘Berel’;the‘Vladivostok’GA3 content was lower than that of‘Berel’in veraison.(3)In ‘Vladivostok’,the activities of Cx andβ-glu increased during the fruit ripening period,and the activities of PG and PME reached the peak at last-veraison.In‘Berel’,Cx activities did not change throughout the fruit growth period;By contrast the activities of PG,PME andβ-glu peaked in fruit early growth stage,then decreased when the fruit matured.(4)In‘Vladivostok’carpopodium abscission zone,there was significantly negative correlation between Cx and IAA/ABA(P<0.05).In‘Berel’carpopodium abscission zone,β-glu activity was significan
作者
刘化禹
娄爽
秦栋
张妍
谢佳璇
霍俊伟
LIU Huayu;LOU Shuang;QIN Dong;ZHANG Yan;XIE Jiaxuan;HUO Junwei(Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region),Ministry of Agriculture/Northeast Agricultural University,College of Horticulture and Landscape Architecture,Northeast Agricultural University,National -Local Joint Engineering Research Center for Development and Utilization of Small Fruits in Cold Regions,Harbin 150030,China)
出处
《西北植物学报》
CAS
CSCD
北大核心
2019年第1期110-120,共11页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家重点研发计划(2016YFC500304-05)
黑龙江省自然科学基金(C2017020)
黑龙江省应用技术研究与开发计划重大项目(GA18B107)
国家自然科学基金(31272130)
关键词
蓝果忍冬
果实脱落
离区
激素
细胞壁相关酶
blue honeysuckle
fruit abscission
abscission zone
hormone
cell wall-related enzymes
