摘要
为探讨Ca^(2+)对百合耐热性的影响及其生理机制,以东方百合杂种系西伯利亚为试验材料,研究CaCl_2、钙离子螯合剂EGTA、钙调蛋白抑制剂三氟拉嗪(TFP)处理对40℃高温胁迫下百合热害指数、抗性相关生理指标和相关热响应基因表达的影响。结果表明,高温胁迫下,15 mmol/L CaCl_2处理能够减缓膜透性的加大、叶绿素的降解和丙二醛的积累,提高脯氨酸、可溶性蛋白含量和超氧化物歧化酶活性,促进热响应基因LoCaM3和LoHsfA3a的表达,从而提高植株的耐热性,降低热害指数。10 mmol/L EGTA和200μmol/L TFP的作用则相反。表明作为植物生长所必需的大量元素,Ca^(2+)也可能通过Ca^(2+)-CaM信使系统提高渗透调节能力、抗氧化能力、光合能力和蛋白质活性,以维持细胞稳态来调节百合对高温逆境的适应性。
To determine the effect and physiological mechanism of calcium on the thermotolerance of lily, the heat injury index,correlative physiological indexes and heat-responsed genes under high temperature stress of 40 ℃ were investigated by treating Lilium oriental Siberia with exogenous Ca^2+, Ca^2+ chelating agent EGTA and calmodulin antagonist TFP.The results showed that under the circumstance of heatstress,15 mmol/L Ca^2+ treatment alleviated leakage of electrolyte, degradation of chlorophyll and the accumulation of MDA caused by heat stress,enhanced the content of proline,soluble protein and activity of SOD,and induced the expression of heat-responsed genes LoCaM3 and LoHsfA3a ,which increased tolerance to heat stress and decreased heat injury index.The role of 10 mmol/L EGTA or 200 μmol/L TFP was opposite. The results indicated that as one essential macroelement towards plant growth,Ca^2+ might regulate the heat resistance of lily through Ca^2+-CaM signal transduction by improving the ability of osmoregulation, antioxidation, photosynthesis and the activity of protein to maintain cellular homeostasis.
作者
张铭顺
吕福堂
褚鹏飞
吴泽
义鸣放
曹兴
ZHANG Mingshun;LV Futang;CHU Pengfei;WU Ze;YI Mingfang;CAO Xing(College of Agriculture,Liaocheng University,Liaocheng 252059,China;College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;College of Horticulture,China Agricultural University/Beijing Key Laboratory ofDevelopment and Quality Control of Ornamental Crops,Beijing 100193,China)
出处
《河南农业科学》
北大核心
2019年第2期120-125,共6页
Journal of Henan Agricultural Sciences
基金
山东省自然科学基金项目(ZR2016CB36)
国家自然科学基金项目(31471904
31601788)
