摘要
针对辽西北地区干旱缺水、土壤贫瘠、造林成活率低等问题,研究在对樟子松接种外生菌根真菌的基础上施加一定浓度的外源Ca^(2+),目的是研究菌根真菌和Ca^(2+)的互作对樟子松根际微环境改良作用。于生长季节,利用盆栽方法,在种子出苗后人工接种外生菌根菌(ECMF)-褐环乳牛肝菌(Suillus luteus),并施用0 mmol L^(-1)~50 mmol L^(-1) CaCl_2,以不接菌和不加CaCl_2为对照。在苗木生长3个月后,分别测定苗木的菌根侵染率、苗木根际土壤过氧化氢酶等土壤酶活性、根际土壤微生物数量、有效磷含量等土壤指标,评价外源钙和外生菌根菌对樟子松根际土壤的改良作用。结果表明:1)适当浓度的外源Ca^(2+)和外生菌根菌协同能够更好的提高苗木根际土壤酶活性,提高根际土壤养分含量。2)适当浓度的外源Ca^(2+)和外生菌根菌协同能够更好的增加樟子松苗木根际微生物数量,增加根际土壤生物活性。3)适当浓度的外源Ca^(2+)和外生菌根菌协同能够更好的促进樟子松苗木的生长。综上,施加外源钙能够达到改良樟子松苗木的根际土壤,提高土壤生物活性的目的。在苗圃育苗初期或者造林时,在接种外生菌根真菌进行菌根化育苗的同时,施加适当浓度的外源钙来实现改良土壤是可行的。
There are some issues of drought and water shortage, soil degradation, and low afforestation survival rate in northwestern Liaoning. In this research, different concentrations of exogenous Ca^2＋afterectotrophic mycorrhiza fungi（ECMF）vaccinationto Pinussylvestrisvar. mongolica were applied for clarifying themutual interactions of ECMF and exogenous Ca^2＋on the improvement of rhizosphere microenvironment. We inoculated ECMF-Suillus luteus after the seed emergence, and then applied 0 mmol L^-1 -50 mmol L^-1 CaCl2 to soil in pot experiments. The control treatments were set as seedlings no ECMF and CaCl2 addition. After 3 months of seedling growth, the colonization rates of seedlings, soil enzyme activities such ascatalase activity, microbial quantity and phosphorus content in rhizosphere soilof Pinus sylvestrisvar. mongolica seedling were determined, and the effects of exogenous calcium and ECMF on rhizosphere soil improvement of Pinus sylvestrisvar. mongolica seedling were evaluated. The results showed that 1）Combined application of appropriate concentration of exogenous Ca^2＋and ECMF improved enzyme activitiesand nutrient contentsin rhizosphere soil of Pinus sylvestrisvar. mongolica seedling. 2）Combined application of appropriate concentration of exogenous Ca^2＋and ECMF better increased microbial quantity and activityin rhizosphere soil of Pinus sylvestrisvar. mongolica seedlings and rhizosphere soil biological activity. 3） Combined application of appropriate concentration of exogenous calcium and ECMF promoted the growth of Pinussylvestrisvar. mongolica. In conclusion,applying appropriate concentration of exogenous calcium indeed improved rhizosphere soil of Pinus sylvestris var.mongolica seedlings and soil biological activity. In the early stages of nursery seedling or afforestation, it is feasible to improvesoilfertility that applying ECMF vaccination combined with appropriate concentration of exogenous calcium.
作者
尹大川
祁金玉
邓继峰
邓勋
YIN Da-chuan;QI Jin-yu;DENG Ji-feng;DENG Xun(College of Forestry, Shenyang Agricultural University, Shenyang 110866, China;Forestry Protection Institute of Heilongjiang Forestry Academy, Harbin 150040, China)
出处
《土壤通报》
CAS
北大核心
2018年第2期362-366,共5页
Chinese Journal of Soil Science
基金
辽宁省教育厅项目(01052017004)
国家自然科学基金项目(31670649)
沈阳农业大学引进人才启动项目(880416021)资助
关键词
外生菌根菌
外源钙
樟子松
土壤改良
Ectomycorrhizal fungi
Exogenous calcium
Pinus sylvestris var. mongolica
Soil improvement
