摘要
该试验以‘平邑甜茶’60 d龄幼苗为材料,设置T1(5000 r/min×3 h)、T2(5000 r/min×6 h)、T3(10000 r/min×3 h)和T4(10000 r/min×6 h)超重力处理,以未进行超重力处理为对照(CK),考察超重力处理下幼苗叶片叶绿素、可溶性蛋白、丙二醛、脯氨酸含量和膜透性等与逆境相关的生理指标的变化,并对其进行转录组分析,初步探究‘平邑甜茶’在超重力处理下的转录组响应机制。结果表明:(1)超重力处理造成‘平邑甜茶’幼苗叶片由舒展状生长状态向不同萎缩生长状态转变,其中T4处理萎缩生长状态明显。(2)超重力处理下,‘平邑甜茶’幼苗叶片叶绿素和丙二醛含量在T3和T4处理下显著高于CK,T1和T2处理下与CK无显著差异;叶片脯氨酸含量在各超重力处理下均显著高于CK;膜透性在T4处理下显著高于CK,T1、T2、T3处理与CK无显著差异;可溶性蛋白含量在各超重力处理下均不同程度降低,但均与CK差异不显著。(3)样品测序结果显示,大多数转录本的序列长度在1000 nt以上,有37725个,占全部转录本79.15%,且CK、T2、T4样品以及相对应的新基因中均以FPKM值小于5.0的转录本数为主,其转录本数依次分别有21412、20162、22368个和1352、1411、1406个,占比分别为45.78%、43.10%、47.82%和81.45%、85.00%、84.70%。(4)GO功能注释与富集分析显示,随着超重力处理强度的加大,差异表达基因在GO富集分类中存在明显不同的途径,说明‘平邑甜茶’对超重力的响应是复杂的,但主要集中在转录因子活性、蛋白质去磷酸化过程方面。(5)差异转录因子表达分析显示,随超重力处理强度的增加表达上调幅度比较大的基因家族有bZIP、WRKY和ERF,其中bZIP和WRKY转录因子家族中的基因表达量均随超重力处理强度的增加呈上升趋势,但ERF家族中的MD01G1177100、MD08G1096000、MD13G1130700基因表达量随超重力处理强度的增加有下降的趋势。研究表明,超重力处理�
In this study,we used 60-day-old seedlings of Malus hupenensis as materials,and sets T1(5000 r/min×3 h),T2(5000 r/min×6 h),T3(10000 r/min×3 h)and T4(10000 r/min×6 h)hyper-gravity stress,with no hyper-gravity stress as the control(CK).We investigated the chlorophyll,soluble protein,malondialdehyde,proline contents and membrane permeability of the seedling leaves under the hyper-gravity stress and transcriptome analysis on them,to preliminary explore the transcriptome response mechanism of M.hupenensis under hyper-gravity stress.The results showed that:(1)the hyper-gravity stress caused the leaves of the M.hupenensis seedlings to change from a stretched growth state to a different atrophy growth state,and the T4 showed a significant atrophy growth state.(2)Under hyper-gravity stress,the chlorophyll and malondialdehyde contents of the seedling leaves of M.hupenensis under T3 and T4 were significantly higher than that of CK,and there was no significant difference between CK,T1 and T2 treatments.The proline content of leaves under all hyper-gravity stresses were significantly higher than that of CK.The membrane permeability under T4 was significantly higher than that of CK,and there was no significant difference between T1,T2,T3 and CK.The soluble protein content is reduced to different degrees under each hyper-gravity stress,but it is not significantly different from CK.(3)The sample sequencing results shows,the sequence length of most transcripts is more than 1000 nt,total of 37725 accounting for 79.15%of all transcripts,and CK,T2,T4 samples and the corresponding new genes are dominated by transcripts with FPKM value<5.0,the number of transcripts were 21412,20162,22368 and 1352,1411 and 1406 respectively,accounting for 45.78%,43.10%,47.82%and 81.45%,85.00%and 84.70%,respectively.(4)GO function annotation and enrichment analysis show that there are obviously different ways of differentially expressed genes in GO enrichment classification,as the intensity of hyper-gravity stress increases.It implies that the respo
作者
刘媛媛
申成丞
吕红柳
吴非
段国锋
LIU Yuanyuan;SHEN Chengcheng;LÜHongliu;WU Fei;DUAN Guofeng(College of Horticulture, Shanxi Agricultural University, Taigu, Shanxi 030801, China)
出处
《西北植物学报》
CAS
CSCD
北大核心
2020年第9期1518-1526,共9页
Acta Botanica Boreali-Occidentalia Sinica
基金
山西省重点研发计划(201703D221028-1)
山西省1331成果转化培育项目(2020CG027)。
关键词
平邑甜茶
超重力
生理指标
转录组
Malus hupenensis hyper-gravity
physiological index
transcriptome
