摘要
应用新一代高通量测序技术(Illumina Solexa),对中麻黄(Ephedra intermedin)同萌期的种子进行转录组测序,数据重头(denovo)组装后,共获得52 007条Unigene序列,总长为25 043 596 bp。COG功能注释、GO分类及KEGG代谢通路分析后,获得了64 751个GO功能注释,17 701个COG功能注释以及16 942个KEGG注释,并从KEGG通路中找到参与黄酮类化合物合成途径的关键基因片段283个,其中包含了查尔酮合酶基因、细胞色素相关基因和花青素还原酶等基因。中麻黄转录组测序工作的完成,极大地扩充了麻黄的基因资源,为麻黄属植物分子系统进化分析、抗性和药用功能基因的发掘与利用、遗传改良等研究奠定基础。
The application of a new generation high-throughput sequencing technology （Illumina solexa） to global transcriptome of different stages of germinated Ephedra intermedia Schrenk ex Mey seeds showed that 52 007 unigenes were acquired,and the total length was 25 043 596 bp after de novo assembly; among them,64 751 annotations were from GO,17 701 from COG and 16 942 from KEGG after functional annotation against these databases; from KEGG pathway,283 unigenes were associated with flavonoids biosynthetic pathway which contained chalcone synthase,cytochrome,anthocyanidin reductase,etc.The result could provide some references for the study of molecular systematic evolution,utilization of resistance and medicine related genes,and its genetic improvement in the future.
出处
《林业科学研究》
CSCD
北大核心
2014年第6期758-763,共6页
Forest Research
基金
中央级公益性科研院所基本科研业务费专项资金(RIF2010-10)
林木遗传育种国家重点实验室专项课题(TGB2013012)
关键词
中麻黄
转录组
黄酮类化合物
高通量测序
Ephedra intermedia
transcriptome
flavonoid
high-throughput sequencing
