摘要
利用BCECF-AM荧光染料和激光共聚焦显微镜测定谷子根部细胞内的荧光强度,进而换算成细胞内pH值,以此来研建测定谷子根部细胞内pH值的方法。结果显示:正常水培条件下,谷子品种YG2和AN04根部细胞内pH值分别为5.85±0.11和5.77±0.26;盐处理下YG2和AN04根部细胞内pH值分别达到6.21±0.26和6.19±0.17,即盐敏感品种AN04根部细胞盐处理后pH值变化较大,提高7.28%,而抗盐品种YG2提高6.15%。这说明抗盐品种盐胁迫下细胞内pH值受外界环境影响较小,对盐害的耐受力较高。该方法用来定量测定细胞内pH值具有可信性,亦可作为评价植物耐受胁迫能力的辅助手段。
The fluorescent probe of BCECF-AM was used to measure the intracellular pH value in root cells of foxtail millet in this research. The fluorescence intensity was detected with laser scanning confocal microscopy, and then was converted to pH value. The results showed that the pH values in root cells of the foxtail millet varieties YG2 and AN04 were 5.85±0.11 and 5.77±0.26 respectively under normal hydroponic culture, but increased to 6.21±0.26 and 6.19±0.17 respectively under salt treatment. The pH value of salt- sensitive variety AN04 increased by 7.2%, which was a little more than that of salt-resistant variety YG2 of 6.15% under salt stress. The results indicated that the intracellular pH value of salt-tolerant varieties was less affected by external environment, and their tolerance to salt damage was higher. This method was reliable for the quantitative determination of intracellular pH value, and it could be used as an auxiliary means to evaluate the tolerance of plants to stress.
作者
李小波
潘教文
丁国华
李臻
王庆国
刘炜
Li Xiaobo;Pan Jiaowen;Ding Guohua;Li Zhen;Wang Qingguo;Liu Wei(Biotechnology Research Center, Shandong Acadamy of Agricultural Sciences/Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan 250100, China;School of Life Sciences and Technology, Harbin Normal University, Harbin 150025, China;College of Life Sciences, Shandong Normal University, Jinan 250014, China)
出处
《山东农业科学》
2019年第2期40-44,共5页
Shandong Agricultural Sciences
基金
山东省自然科学基金面上项目(ZR2016CM23)
山东省自然科学基金青年基金项目(ZR2014CQ24)
山东省农业科学院青年英才计划项目(2016-2018)
山东省农业科学院青年基金项目(2014QNM37)
关键词
谷子
盐胁迫
BCECF-AM荧光染料
根部细胞内pH值
Foxtail milliet (Setaria italic L.)
Salt stress
BCECF-AM fluorescent dye
Intracellular pH value in root
