摘要
目的探讨川芎嗪(tetramethylpyrazine,TMP)对庆大霉素(gentamycin,GM)耳毒性作用的防治机理。方法将36只豚鼠随机分成生理盐水组、庆大霉素造模组、川芎嗪防治组,每组各12只。从病理层面研究,利用TUNEL法标记比较3组豚鼠耳蜗螺旋神经节(spiralganglioncells,SGC)、螺旋器及侧壁(lateralwall,LW)内细胞凋亡情况。从基因水平研究,利用Real-timePCR法检测C-Jun氨基末端激酶(c-jun n-terminal kinases,JNK)的mRNA含量。从听力学角度,结合听性脑干反应(auditory brainstem response,ABR)测试观察用药前后豚鼠听力的变化。结果与川芎嗪防治组相比,庆大霉素造模组的耳蜗螺旋神经节细胞凋亡增多(P<0.05),螺旋器和侧壁细胞凋亡明显增多(P<0.001),JNK的mRNA表达明显升高(P<0.05),同时ABR阈移显著增大(P<0.01)。结论川芎嗪可明显降低庆大霉素导致的JNK转录水平上的高表达,从而有效防护庆大霉素的耳毒性,这可能是川芎嗪发挥防护作用的机制之一。
Objective To identify the protection mechanism of Tetramethy PYrazine in the intervention of Gentamic in ototoxicity.Methods Thirty-six guinea pigs were randomly divided into 3 groups,12 in each:physiological saline group,gentamicin-made module and tetramethylpyrazine prevention group.From the pathological level,the TUNEL method was used to compare the apoptosis of cochlear spiral ganglion(SGC),spiral organ and lateral wall(LW)in the three groups of the guinea pigs.From the gene level study,the mRNA content of JNK was detected by Real-time PCR.Audiology combined with auditory brainstem response(ABR)test was used to observe the changes of hearing in the guinea pigs before and after medication.Results Compared with tetramethylpyrazine prevention group,the apoptosis of the spiral ganglion cells of the gentamicin-made group increased(P<0.05),and the apoptosis of the spiral and sidewall cells increased significantly(P<0.001).The expression of JNK mRNA was significantly increased(P<0.05),while the threshold shift of ABR was significantly increased(P<0.01)Conclusion Tetramethylpyrazine can significantly reduce the high expression of gentamicin-induced JNK transcription level,thus effectively protecting the ototoxicity of gentamicin,which may be one of the mechanisms of the protective effect of tetramethylpyrazine.
作者
王晨露
王永华
王一鸣
郑华平
李文靖
WANG Chen-Lu;WANG Yong-Hua;WANG Yi-Ming;ZHENG Hua-Ping;LI Wen-Jing
出处
《中国听力语言康复科学杂志》
2019年第1期9-12,共4页
Chinese Scientific Journal of Hearing and Speech Rehabilitation
基金
国家自然科学基金项目(63411140)
关键词
庆大霉素
耳毒性
川芎嗪
细胞凋亡
基因
Gentamicin
Ototoxicity
Tetramethylpyrazine
Apoptosis
Gene
