摘要
本文研究了肉桂等精油对多重耐药鲍曼不动杆菌(MDRAB)的体外抑菌效果,采用药敏纸片扩散法和微量肉汤稀释法测定其对MDRAB的抑菌圈直径和最小抑菌浓度(MIC),筛选出抑制效果最好的精油和绘制出该精油对MDRAB的动态杀菌曲线。用棋盘稀释法研究MIC值最小的两种精油联合抗MDRAB的效果。结果表明肉桂精油对MDRAB的抑菌效果最强,抑菌圈直径为29.30±1.16 mm,MIC值为0.62μL/mL,且对MDRAB的抑菌活性存在浓度依赖性,另外肉桂精油与薰衣草精油联合抗MDRAB有相加作用,与薄荷精油联合抗MDRAB有部分协同作用;薰衣草精油和薄荷精油也有较强抑菌效果,MIC值依次为1.25μL/mL和2.50μL/mL;丁香精油对MDRAB有中度抑菌活性,MIC值为10μL/mL;冬青精油和绿茶精油对MDRAB的抑菌作用相对较弱,MIC值为20μL/mL;安息香精油对MDRAB的抑制作用很弱甚至无抑制作用。因此,肉桂精油对MDRAB的抑菌效果最强,且对MDRAB的抑菌活性与浓度正相关,另外与薰衣草精油和薄荷精油有联合抗MDRAB作用,为临床用药提供有效指导。
To observe the antibacterial effect of essential oils on multidrug-resistant Acinetobacter baumannii(MDRAB)in vitro,the inhibitory zone diameter and the minimum inhibitory concentration(MIC)of seven essential oils on MDRAB were measured by disk diffusion method and micro-broth dilution method,the strongest antibacterial essential oil was found and then its dynamic germicidal curve against MDRAB was drawn.The combinatory effect of the two lowest MIC essential oils on MDRAB was studied by the checkerboard dilution method.The results showed that cinnamon essential oil has the strongest antibacterial effect on MDRAB.The inhibition zone diameter is 29.30±1.16 mm,the MIC value is 0.62μL/mL,and the antibacterial activity against MDRAB is dependent on the drug concentration.A synergistic effect was observed with the combination of cinnamon oil with peppermint oil.Additive effect was observed with the combination of cinnamon oil and lavender oil.Furthermore,peppermint oil and lavender oil had stronger antibacterial effect,MIC value were 1.25μL/mL and 2.50μL/mL,respectively.Clove oil had moderate antibacterial activity on MDRAB,the MIC value was 10μL/mL.The antibacterial effect of wintergreen oil and green tea oil on MDRAB were relatively weak,the MIC value was 20μL/mL.The inhibitory effect of benzoin essential oil on MDRAB was weak or not detected.It was concluded that cinnamon oil had the strongest antibacterial effect on MDRAB and was concentration-dependent.In addition,cinnamon essential oil and lavender essential oil as well as cinnamon essential oil and peppermint essential oil had synergistic anti-MDRAB effect,providing effective guidance for clinical use.
作者
岳聪聪
李钰乐
郝小康
郭瑞林
YUE Cong-cong;LI Yu-le;HAO Xiao-kang;GUO Rui-lin(Department of Medical Technology,Shaanxi University of Chinese Medicine,Xianyang 712046,China;Clinical lab,the Second Affiliated Hospital of Shanxi University of Chinese Medicine,Xianyang 712000,China)
出处
《现代食品科技》
EI
CAS
北大核心
2019年第1期109-113,30,共6页
Modern Food Science and Technology
关键词
肉桂精油
MDRAB
杀菌曲线
抑菌作用
cinnamon essential oil
multi-drug resistant Acinetobacter baumannii
concentration-killing curve
antibacterial effect
