摘要
目的研究Mindin蛋白在CCl4诱导的小鼠急性肝损伤过程中的动态表达情况,并探讨其作用机制。方法雄性C57B1/6小鼠48只,随机抽取40只应用CCl4橄榄油溶液腹腔注射诱导急性肝损伤,分别于造模后6、12、24、48和72 h取小鼠肝脏组织作病理学观察(实验组),余下8只腹腔注射橄榄油作为对照(对照组)。应用Western Blot法检测Mindin蛋白的表达以及变化趋势,实时荧光定量PCR检测肝组织Mindin mRNA的表达。计量资料两组间比较采用t检验。结果 CCl4诱导肝损伤后,小鼠肝脏失去其正常结构,48 h时肝脏病理改变最为显著。肝脏Mindin蛋白在注射CCl4后12～72 h内呈现低表达水平,而其转录水平即Mindin mRNA在给予CCl4后12 h降至最低(实验组vs对照组:0. 183±0. 105 vs 1. 023±0. 247,t=8. 841,P <0. 01),随后逐渐升高,在48 h(2. 548±0. 775 vs 1. 023±0. 247)和72 h(2. 699±0. 995 vs 1. 023±0. 247)时转录水平甚至是正常水平的2倍以上,差异均有统计学意义(t值分别为5. 428、4. 621,P值均<0. 01)。结论 CCl4诱导小鼠急性肝损伤过程中,Mindin蛋白显著变化,表现为蛋白表达下调,以及转录后mRNA水平的调节。提示Mindin蛋白在CCl4诱导急性肝损伤过程中可能发挥重要作用。
Objective To investigate the dynamic expression of Mindin protein in mice with acute liver injury induced by carbon tetrachloride(CCl 4)and its mechanism of action.Methods A total of 48 male C57B1/6 mice were selected and divided into experimental group(n=40)and control group(n=8).The mice in the experimental group were given intraperitoneal injection of CCl 4 olive oil to induce acute liver injury,and the liver tissue was collected for pathological observation at 6,12,24,48,and 72 hours after modeling.The mice in the control group were given intraperitoneal injection of olive oil.Western blot was used to measure the protein expression of Mindin and its change trend,and quantitative real-time PCR was used to measure the mRNA expression of Mindin.The t-test was used for comparison of continuous data between two groups.Results Abnormal liver structure was observed after liver injury was induced by CCl 4,with the most significant pathological change at 48 hours.There was a low protein expression level of Mindin within 12-72 hours after the injection of CCl 4.The mRNA expression of Mindin reached the lowest level at 12 hours after CCl 4 injection and there was a significant difference between the experimental group and the control group at this time point(0.183±0.105 vs 1.023±0.247,t=8.841,P<0.01);then there was a gradual increase in the mRNA expression of Mindin,and at 48 and 72 hours,the mRNA expression of Mindin in the experimental group was more than 2 times that in the control group(48 hours:2.548±0.775 vs 1.023±0.247,t=5.428,P<0.01;72 hours:2.699±0.995 vs 1.023±0.247,t=4.621,P<0.01).Conclusion There is a significant change in Mindin protein during the process of acute liver injury induced by CCl 4 in mice,which is characterized by the downregulation of protein expression and the regulation of post-transcriptional mRNA level,suggesting that Mindin may play an important role in the process of acute liver injury induced by CCl 4.
作者
张欣
王文俊
翟嵩
李亚萍
石娟娟
贾晓黎
党双锁
ZHANG Xin;WANG Wenjun;ZHAI Song(Department of Infectious Diseases,The Second Affiliated Hospital of Xi′an Jiaotong University,Xi′an 710004,China)
出处
《临床肝胆病杂志》
CAS
北大核心
2019年第2期368-371,共4页
Journal of Clinical Hepatology
基金
国家自然科学基金项目(81500453)
陕西省社发科技攻关项目(S2015YFSF0363)
关键词
急性肝损伤
细胞外基质蛋白质类
小鼠
近交系
acute liver injury
extracellular matrix proteins
mice,inbred strains
