摘要
本研究建立在对金手指葡萄果形转录组分析的基础上,通过分析样品间表达差异发现,与对照相比,GA20ox2基因的转录水平在GA3处理后有显著差异。为了进一步从分子水平阐述GA20ox2在赤霉素合成途径中的作用机理,本研究以金手指葡萄幼果为材料,克隆了GA20ox2基因,分析了此基因碱基序列特征,并通过构建融合表达载体对其进行亚细胞定位分析。研究结果表明,克隆了葡萄赤霉素合成途径中的VvGA20ox2基因,测序得CDS全长为1 134 bp,编码377个氨基酸。VvGA20ox2编码的氨基酸序列与NCBI上其他物种GA20ox序列相似性在51%～67%,其中该基因与可可(EOX92603.1)和杨树(PNT28192.1)的亲缘关系较近。该基因定位在细胞核和细胞膜中。外源GA3处理后的成熟葡萄果实与对照相比纵径显著伸长,且在幼果中该基因表达量在一段时间内明显上升,推测VvGA20ox2基因可能参与葡萄果形拉长的调控。
In this study,based on the transcriptome analysis of grape berry shape change,it was found that there was a significant expression difference among samples in the transcriptional level of the GA20ox2 gene after gibberellin(GA3)treatment compared with the control.In order to study molecular mechanism of GA20ox2 in GA biosynthesis pathway,VvGA20ox2 gene was cloned from Golden Finger grape.The base sequence characteristics of GA20ox2 gene were analyzed,and subcellular localization analysis was performed by constructing a fusion expression vector.The results showed that the VvGA20ox2 gene related to gibberellin synthesis pathway was successfully cloned,and the total length of CDS was 1 134 bp,and 377 amino acids were encoded.The sequence similarity of VvGA20ox2 to other nine species GA20ox protein sequences in NCBI was 51%-67%,it had closer relationship with cacao(EOX92603.1)and poplar(PNT28192.1).The VvGA20ox2 gene was located in both the plasma membrane and nucleus.The longitudinal diameter of mature grape berries treated with GA 3 was significantly longer than that of the control,and the expression level of VvGA20ox2 gene in young fruit increased significantly.It is speculated that the VvGA20ox2 gene may be involved in the regulation of grape shape.
作者
高世敏
董阳
王武
陶建敏
GAO Shi-min;DONG Yang;WANG Wu;TAO Jian-min(College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China)
出处
《江苏农业学报》
CSCD
北大核心
2018年第6期1331-1338,共8页
Jiangsu Journal of Agricultural Sciences
基金
国家现代农业葡萄产业技术体系项目(CARS-29)
国家"948"重点项目(2016-X19)
江苏省农业重大新品种创制项目(PZCZ201723)
关键词
葡萄
赤霉素
VvGA20ox2
亚细胞定位
表达分析
Vitis vinifera
gibberellin(GA3)
VvGA20ox2
subcellular localization
expression analysis
