摘要
目的建立免疫透射比浊法自建检测系统钩状效应性能的确认方法。方法采用AU5400全自动生化分析仪,选取3种非配套免疫透射比浊法IgG试剂盒(试剂A、试剂B、试剂C),通过对不同浓度IgG样本进行检测,绘制Heidel-berger曲线,确定平衡点及安全范围。比较不同IgG试剂从抗原适量到过量的过程中,快速反应期内吸光度(A)值变化速率、达到峰值时间及上升幅度的差异,确认3种非配套IgG试剂钩状效应的识别参数。结果 3种非配套IgG试剂(试剂A、试剂B、试剂C)的Heidel-berger曲线平衡点浓度分别为49.0、42.0、105.0 g/L;安全范围分别为35.0～90.0、20.0～75.0、33.0～120.0 g/L。试剂A、试剂B、试剂C钩状效应报警的浓度范围分别为49～350、28～70、42～350 g/L。结论免疫透射比浊法自建检测系统可通过钩状效应性能确认方法建立相关识别参数,以确保检测结果的准确、可靠。
Objective To establish the method of hook effect performance verification in laboratory-developed turbidimetric immunoassay systems.Methods A total of3laboratory-developed turbidimetric immunoassay systems(Reagent A,Reagent B and Reagent C)were used for IgG with different levels.Heidel-berger curves were drawn to determine equilibrium points and safe ranges.The changes of absorbance(A),peak times and rising ranges in the rapid reaction period were observed to investigate the recognition parameters of hook effect.Results The equilibrium points of Heidel-berger curves of Reagent A,Reagent B and Reagent C were49.0,42.0and105.0g/L,respectively,and the safe ranges were35.0-90.0,20.0-75.0and33.0-120.0g/L,respectively.The hook effects of Reagent A,Reagent B and Reagent C can be identified in49-350,28-70and42-350g/L,respectively.Conclusions The recognition parameters of laboratory-developed turbidimetric immunoassay systems can be set up by performance verification of hook effect to ensure the accuracy and reliability of results.
作者
夏勇
薛灏
李明洋
纪玲
XIA Yong;XUE Hao;LI Mingyang;JI Ling(Department of Clinical Laboratory,Peking University Shenzhen Hospital,Shenzhen 518036,Guangdong,China)
出处
《检验医学》
CAS
2018年第12期1132-1135,共4页
Laboratory Medicine
关键词
钩状效应
性能确认
免疫透射比浊法
Hook effect
Performance verification
Turbidimetric immunoassay
