摘要
为了解猪禽源大肠埃希菌和沙门菌对黏菌素的耐药性以及MCR-1基因的流行情况,选取2008年-2015年采集的18 203株大肠埃希菌和2009年-2015年采集的1 729株沙门菌为研究对象,使用WPS Office 2016软件对菌株的MIC值分布和耐药结果进行统计分析,用PCR对部分菌株进行MCR-1基因检测。统计发现,在MIC≥4μg/mL时,大肠埃希菌和沙门菌所占比例分别为17.97%和29.38%;而EUCAST的结果分别为1.84%和5.63%,在耐药浓度范围内,大肠埃希菌和沙门菌均在MIC值为4μg/mL时所占比例最高,分别为15.93%和21.86%。自2013年两种菌开始出现MIC>16μg/mL的菌株。大肠埃希菌和沙门菌对黏菌素的耐药率分别为17.97%和29.38%,猪禽源大肠埃希菌和沙门菌耐药率分别为24.25%、12.3%和17.26%、38.94%。MCR-1基因检出率为11.73%,大肠埃希菌检出率比沙门菌高15.39%,MIC值为4μg/mL时阳性菌株分布最多(72.18%),2008年-2015年阳性菌株比例分别为0.19%、5.26%、4.68%、30.99%、12.28%、12.67%、14.81%、19.10%。大肠埃希菌和沙门菌对黏菌素耐药率相对不高,多数为MIC=4μg/mL的低水平耐药且逐渐出现了MIC>16μg/mL的高水平耐药菌,与EUCAST相比,其MIC值分布相对后移。沙门菌对黏菌素的耐药率高于大肠埃希菌,但后者耐药日趋严重,不同动物源菌株对黏菌素的耐药程度存在差异,猪源大肠埃希菌和禽源沙门菌耐药程度相对严重。MCR-1检出率较低,但呈现增长趋势,该基因主要引起MIC=4μg/mL的低水平耐药且主要在大肠埃希菌中被检出。
In order to understand current status on colistin resistance in Escherichia coli and Salmonella isolates from swine and poultry and the prevalence of mcr-1in China,we analyzed the MIC distribution and drug-resistance with18203Escherichia coli isolates collected in2008-2015and1729Salmonella isolates collected in2009-2015by WPS office2016software,and tested the mcr-1gene of some strains by PCR.The results showed that the proportion of Escherichia coli and Salmonella isolates was17.97%and29.38%respectively when MIC value was no less than4μg/mL,while that of EUCAST was1.84%and5.63%respectively.The proportion of Escherichia coli and Salmonella isolates hit the highest level(15.93%and21.86%respectively)with MIC value being4μg/mL.Since2013,Escherichia coli and Salmonella strains with MIC values higher than16μg/mL appeared.The resistance rates of Escherichia coli and Salmonella isolates to colistin were17.97%and29.38%respectively,of which the resistance rates of Escherichia coli isolated from swine and poultry were24.25%and12.3%,those of Salmonella isolated from swine and poultry were17.26%and38.94%,respectively.The detection rate of mcr-1gene was11.73%.The proportion of mcr-1positive strains in Escherichia coli was15.39%higher than that in Salmonella.The distribution of positive strains was the highest when MIC value was4μg/mL(72.18%).The proportion of mcr-1positive strains from2008to2015was0.19%,5.26%,4.68%,30.99%,12.28%,12.67%,14.81%and19.10%,respectively.The resistance rates of Escherichia coli and Salmonella isolates to colistin were relatively low,most of which were low-level resistant strains(MIC=4μg/mL)and high-level resistant strains(MIC>16μg/mL)gradually appeared.Compared with EUCAST,the proportion of isolates with MIC value being no less than4μg/mL was relatively high.The resistance rate of Salmonella isolates to colistin was higher than that of Escherichia coli isolates,but the AMR problem for the latter one is becoming increasingly serious.The resistance rate to colistin of isolates from different ani
作者
李振斌
姜雯
宋传周
赵建梅
李月华
迟良
刘焕奇
曲志娜
LI Zhen-bin;JIANG Wen;SONG Chuan-zhou;ZHAO Jian-mei;LI Yue-hua;CHI Liang;LIU Huan-qi;QU Zhi-na(China Animal Health and Epidemiology Center,Qingdao,Shandong,266032,China;Department of Animal Science,Qingdao Agricultural University,Qingdao,Shandong,266109,China;Pingyuan Animal Husbandry and Veterinary Bureau Wangmiao Animal Inspection Branch,Dezhou,Shandong,253100,China)
出处
《动物医学进展》
北大核心
2018年第12期20-26,共7页
Progress In Veterinary Medicine
基金
国家"十二五"科技基础性工作专项(2012FY111000)
