摘要
禽致病性大肠埃希菌(avian pathogenic Escherichia coli,APEC)的毒力基因较为复杂,其中ETT2(Escherichia coli type three secretion system 2)毒力岛在禽源大肠埃希菌鲜有报道。选择ETT2两端的ECs3703和ECs3737作为检测标志基因,参照文献合成2对引物,建立Duplex PCR方法用于快速检测携带ETT2的禽源大肠埃希菌。通过对参考菌株的扩增,建立特异的Duplex PCR快速检测方法。通过对187份临床样品的检测,发现其中77份(41.2%)样品存在ETT2阳性大肠埃希菌感染;通过对247株禽源大肠埃希菌临床分离株的检测,发现46.9%的禽源大肠埃希菌携带ETT2毒力岛。
The virulence genes of avian pathogenic Escherichia coli（APEC） are more complex,especially the pathogenicity island ETT2 is rarely reported in recent research.In this study,the ECs3703 and ECs3737 genes were used as the markers for the detection of the ETT2 element,and a Duplex PCR method was established to rapid detect the ETT2-harboring avian E.coli.The specificity of the PCR was confirmed by using reference strains.187 samples from diseased poultry were submitted to Duplex PCR detection,and the results revealed that 77（41.2%） were infected with ETT2-harboring avian E.coli.A total of 247 bacteria isolates were made further identified by the PCR method,and the data showed that 116（46.9%） isolates were positive for ETT2 pathogenicity island.
出处
《扬州大学学报（农业与生命科学版）》
CAS
北大核心
2017年第4期14-17,共4页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
江苏现代农业(水禽)产业技术体系集成创新中心项目(SXGC[2017]248)
江苏省六大人才高峰项目(2014-NY-023)
