摘要
In this study, 68 fresh pork samples were collected from several major wholesale markets in Dalian City of Liaoning Province to isolate Salmonella using SS agar medium as selective medium. Salmonella isolates were identified with colony morphology observation and PCR method. The resistance of Salmonella iso- lates to 15 antibiotics was analyzed by broth microdilution susceptibility test. Accord- ing to the result, 12 Salmonella strains were isolated from 68 fresh pork samples, indicating a detection rate of 17.64%; 83.33% of Salmonella strains isolated from fresh pork samples could at least resist one antibiotic; Salmonella strains exhibited the strongest resistance to florfenicol （75.0%）, sarafloxacin hydrochloride （66.7%）, neomycin sulfate （66.7%） and doxycycline hydrochloride （66.7%）, followed by oxyte- tracycline （58.3%）, gentamicin sulphate （58.3%） and mequindox （58.3%）; Salmonella strains exhibited relatively low resistance to ciprofloxacin lactate （50.0%）, en- rofloxacin （50.0%）, ciprofloxacin hydrochloride （50.0%）, amoxicillin （16.7%） and col- istin sulphate （16.7%）. Salmonella in fresh pork samples from wholesale markets in Dalian Economic ＆ Technological Development Zone exhibited a high detection rate, and the isolated Salmonella strains showed extremely high resistance to various an- tibiotics. The strong drug-resistance of Salmonella can also be transmitted through the food chain from the food to the people, which should attract sufficient attention.
采集辽宁省大连市各大农贸批发市场鲜猪肉68份,用选择性培养基SS琼脂培养基分离沙门氏菌,通过菌落形态及PCR方法对分离菌株进行鉴定,利用肉汤微量稀释法药敏试验检测沙门氏菌对9大类15种抗生素的耐药性。结果表明:共获得鲜猪肉样品沙门氏菌12株,检出率为17.64%;83.33%鲜猪肉样品沙门氏菌至少耐受1种抗菌药,耐药率最高的为氟苯尼考(75.0%)、盐酸沙拉沙星(66.7%)、硫酸新霉素(66.7%)和多西环素(66.7%),其次为土霉素(58.3%)、硫酸庆大霉素(58.3%)、乙酰甲喹(58.3%),耐药率较低的有乳酸环丙沙星(50.0%)、恩诺沙星(50.0%)、盐酸环丙沙星(50.0%)、阿莫西林(16.7%)、硫酸黏菌素(16.7%);大连市农贸批发市场鲜猪肉的沙门氏菌检出率较高,并且沙门氏菌对各类抗生素表现出了很高的耐药性,这种高耐药性也可能通过食物链传递给人类,应该引起足够重视。
基金
Supported by Science and Technology Project of Dalian City(2014B11NC094)
Science and Technology Project of Jinzhou New District of Dalian City(KJCX-ZTPY-2014-0013)
Independent Fund for the Central Universities of China(DC2015010020401)
Key Project in the National Science&Technology Pillar Program during the Twelfth Fiveyear Plan Period(2012BAD38B05)~~
