摘要
为研究丝状支原体山羊亚种Mmc-3740基因编码蛋白的结构与功能,运用生物信息学软件,对该基因编码蛋白的二级结构、跨膜区域、信号肽等生物信息进行预测及分析。结果显示,该基因编码蛋白拥有α螺旋、延伸链、随机卷曲结构;有信号肽,亚细胞定位在细胞外或者细胞质内,可能为分泌蛋白,具有较高的免疫原性,抗原表位偏高的区域主要集中在氨基酸序列的第28~51、60~67、80~130位处;蛋白分子式为C607H1000N184O261S5,分子量15.21kD,等电点(PI值)为4.42,在微生物体内半衰期较短,为不稳定蛋白。该结果为Mmc-3740编码蛋白的进一步研究提供基础数据。
Structure and function of Mmc-3740 encoded protein of Mycoplasma mycoides subsp.capri were studied based on the bioinformatics of the secondary structure,transmembrane region and signal peptide of the gene.The results showed that the protein was an alpha helix with extended random coil,and that the signal peptide located either extracellular or in cytoplasmic with high antigen index and immunogenicity.The area that showed high antigen index and immunogenicity concentrated in the regions of 28-51,60-67 and 80-130 amino acid sequences.The molecular formula of the protein was C 607 H 1000 N 184 O 261 S 5 with a molecular weight of 15.21 kD and an isoelectric point of 4.42.The half-life of Mmc-3740 in microorganisms appeared to be short and unstable.
作者
张靖鹏
江锦秀
林裕胜
游伟
胡奇林
ZHANG Jing-peng;JIANG Jin-xiu;LIN Yu-sheng;YOU Wei;HU Qi-lin(Institute of Animal Husbandry&Veterinary Medicine,Fujian Academy of Agricultural Sciences, Fuzhou,Fujian 350013,China)
出处
《福建农业学报》
CAS
北大核心
2018年第9期883-887,共5页
Fujian Journal of Agricultural Sciences
基金
国家重点研发计划项目(2016YFD0500906)
福建省农业科学院科技创新团队建设项目(2017-09)
福建省农业科学院青年人才创新基金(YC20180-3)
