摘要
大豆胞囊线虫(HeteroderaglycinesIchinohe)是严重危害世界范围大豆生产的害虫,采用合理轮作和种植抗病品种可有效控制损失。为了开展分子标记辅助选择以加速抗病品种培育,本研究针对前期发现的与大豆胞囊线虫3号小种(SCN3)抗性显著关联的非同义变异SNP位点Map-5149,开发高通量、低成本的新型分子标记—竞争性等位基因特异PCR标记(kompetitive allele specific PCR, KASP), GmSNAP11-5149。利用GmSNAP11-5149鉴定了来自8个国家的202份代表性大豆抗感资源,发现141份材料携带抗病基因型GmSNAP11-5149-AA,平均雌虫指数为6.2%,极显著低于58份携带感病基因型GmSNAP11-5149-GG材料的雌虫指数(61.1%),方差分析表明, GmSNAP11-5149与胞囊线虫的抗性显著相关(F=44.18,P<0.0001),对抗病材料的选择效率达到92%,GmSNAP11-5149可作为一个实用的分子标记应用于辅助抗大豆胞囊线虫品种选育和抗病种质资源鉴定。
Soybean cyst nematode(Heterodera glycines Ichinohe)is one of the most economically destructive pathogens worldwide.The cultivation of resistant soybean cultivars combined with crop rotation was the most effective management strategy to reduce yield losses due to SCN.To accelerate the breeding of cultivars resistant to SCN under molecular marker assisted selection,we developed an economical,time-saving and high-throughput KASP marker GmSNAP11-5149 by using a reported non-synonymous polymorphism(Map-5149)on the minor SCN3-11 resistant to SCN3.Two hundred and two soybean accessions from eight countries with known SCN3 were genotyped for GmSNAP11-5149.The average female index of 141 accessions carrying GmSNAP11-5149-AA(6.2%)was significantly lower than that of 58 accessions carrying GmSNAP11-5149-GG(61.1%)at the P<0.0001 probability level.The selection efficiency of GmSNAP11-5149 for resistant accessions was 92%.It is suggested that GmSNAP11-5149 could be used to effectively distinguish resistant genotypes from susceptible ones in the tested soybean accessions and subsequently could be used in molecular marker-assisted breeding and identification of resistant germplasm.
作者
田宇
杨蕾
李英慧
邱丽娟
TIAN Yu;YANG Lei;LI Ying-Hui;QIU Li-Juan(National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Soybean Biology in Beijing(MOA)/Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China)
出处
《作物学报》
CAS
CSCD
北大核心
2018年第11期1600-1611,共12页
Acta Agronomica Sinica
基金
"十三五"国家重点研发计划项目(2016YFD0100201)
国家大豆种质资源平台(2016-004)项目资助~~
