摘要
目的探讨长链非编码RNA肺腺癌转移相关转录物1(lncRNA MALAT1)在骨肉瘤细胞中的表达变化及其对骨肉瘤细胞侵袭能力的影响。方法 (1)采用qRT-PCR法检测骨肉瘤MG-63细胞及正常成骨细胞系h FOB1.19中的lncRNA MALAT1表达。(2)将MG-63细胞随机分为MALAT1沉默组和MALAT1非沉默组,分别转染MALAT1-siRNA质粒及con-siRNA质粒;转染48 h,采用qRT-PCR法检测lncRNA MALAT1表达,Transwell侵袭实验检测细胞侵袭能力,Western blotting法检测ROCK1蛋白表达。(3)将MG-63细胞随机分为对照组、MALAT1沉默组、MALAT1沉默+ROCK1对照组及MALAT1沉默+ROCK1过表达组,分别转染con-siRNA质粒、MALAT1-siRNA质粒、MALAT1-siRNA+pcDNA质粒、MALAT1-siRNA+oe-ROCK1质粒;转染48 h检测各组ROCK1蛋白表达和细胞侵袭能力。结果 (1)MG-63细胞lncRNA MALAT1相对表达量高于hFOB 1.19细胞(P<0.01)。(2)MALAT1沉默组lncRNA MALAT1相对表达量、细胞侵袭能力及ROCK1蛋白相对表达量均低于MALAT1非沉默组(P均<0.01)。(3)对照组、MALAT1沉默+ROCK1过表达组ROCK1蛋白表达及细胞侵袭能力均高于MALAT1沉默组、MALAT1沉默+ROCK1对照组(P均<0.01)。结论骨肉瘤细胞lncRNA MALAT1表达升高;lncRNA MALAT1可能通过调控ROCK1表达而参与骨肉瘤细胞侵袭。
To observe the effect of long non-coding RNA(lncRNA)metastasis-associated lung adenocarcinoma transcript 1(MALAT1)on the cell invasion of osteosarcoma(OS)cells and its possible mechanism.Methods The expression of MALAT1 in the osteosarcoma cell line MG-63 and the normal osteoblast cell line hFOB 1.19 was determined by qRT-PCR.OS cells were divided into the MALAT1-silenced group(transfection of MALAT1-siRNA)and control group(transfection of con-siRNA).At 48 hours after transfection,MALAT1 in each group was measured by qRT-PCR,the metastatic ability of OS cells was determined by Transwell assay,and ROCK1 protein was detected by Western blotting.OS cells were randomly divided into the control group(transfection of con-siRNA),MALAT1 silence group(transfection of MALAT1-siRNA),MALAT1 silence+ROCK1 control group(co-transfection of MALAT1-siRNA and pcDNA),and MALAT1 silence+ROCK1 overexpression group(co-transfection of MALAT1-siRNA and oe-ROCK1).At 48 hours after transfection,the expression of ROCK1 and the metastatic ability in each group were measured.Results The relative expression of lncRNA MALAT1 in the MG-63 cells was higher than that in the hFOB 1.19 cells(P<0.01).The relative expression of lncRNA MALAT1,cell invasive ability and relative expression of ROCK1 protein in the MALAT1-silenced group were lower than those in the control group(all P<0.01).The control group,MALAT1 silence+ROCK1 overexpression group had higher ROCK1 protein expression and cell invasion ability than the MALAT1 silence group,MALAT1 silence+ROCK1 control group(both P<0.01).Conclusion The elevated lncRNA MALAT1 is expressed in OS cells,and lncRNA MALAT1 might participate in the OS cell invasion through ROCK1 expression.
作者
王涛
刘一泽
冯健洲
周禹伯
申德伟
王勇
WANG Tao;LIU Yize;FENG Jianzhou;ZHOU Yubo;SHEN Dewei;WANG Yong(Central Hospital Affiliated to Shenyang Medical College,Shenyang 110024,China)
出处
《山东医药》
CAS
2018年第32期1-4,共4页
Shandong Medical Journal
基金
辽宁省高等学校创新人才支持计划项目(LR2017056)
