摘要
为探讨花椰菜花梗颜色的遗传基础,采用高通量测序技术(Illumina Hi Seq 4000)对青梗和白梗花椰菜进行转录组测序,共获得52 253 822个序列读取片段(reads),对测序的结果从头组装获得66 450个单基因(Unigene),N50为1 285 bp,平均长度为717.40 bp。转录物注释结果显示,45 390个基因有同源比对信息;21 060个基因无匹配序列信息,可能为花椰菜特异的基因序列。对所得差异基因(DEGS)进行不同数据库注释,GO注释到2 540个DEGS,分为细胞组分、分子功能及生物学过程3大类54个功能组;COG注释到的753个Unigene功能系统分为24类;以KEGG数据库为参考,将946个DEGS定位到119个代谢途径分支,注释到6个差异基因与类胡萝卜合成途径有关,9个DEGS与类黄酮生物合成途径有关,1个DEGS与叶绿素代谢相关,且与花椰菜的花梗颜色形成密切相关。16个差异基因通过qRT-PCR验证,结果与RNA-Seq测序结果一致。本研究结果进一步揭示花椰菜花梗颜色的形成机理,为花椰菜品种遗传改良奠定了一定的理论基础。
In order to explore the genetic basis for the colour of cauliflower curd stalk formation,the green stalk and the white stalk were sequenced by Illumina Hi Seq 4000 platform,a total of 52 253 822 readings were generated. And 66 450 unigenes with 1 285 bp length of N50,were obtained by denovo assembly method,the average unigenes length was717. 40 bp. Annotation analysis of unigene indicated that 45 390 unigenes had homologes in public protein database;However,21 060 sequences had no hits and might be specific in Brassica oleracea L. var. botrytis L. specific. All DEGS were annotated by different databases. By GO database,the 2 540 DEGS were divided into 3 categories containing 54 function groups; by COG databases,753 DEGS were grouped into 24 functional categories; By KEGG database,946 DEGS were divided into 119 metabolism pathways,among which 6 DEGS which were involved in Carotenoid biosynthesis pathway,9 DEGS may take part in Flavonoid biosynthesis pathway and 1 DEGS may take part in chlorophyll metabolism pathway and vesponsible for the colour formation. 16 DEGS were verified by qRT-PCR,and showed consistent with the results of RNA-Seq sequencing. These findings provide a scientific basis for further revealing the mechanism of the colour in cauliflower stalk and lay a theoretical foundation for the genetic improvement on cauliflower.
作者
林珲
薛珠政
李永平
李大忠
刘建汀
朱海生
温庆放
XUE Zhuzheng;LI Yongping;LI Dazhong;LIU Jianting;ZHU Haisheng;WEN Qingfang(Fujian Engineering Research Center for Vegetables~Vegetable Research Center,Fujian Academy of Agricultural Sciences~Crops Research lnstitute,Fufian Academy of Agricultural Science;Fuzhou,Fufian 35001)
出处
《核农学报》
CAS
CSCD
北大核心
2018年第9期1708-1720,共13页
Journal of Nuclear Agricultural Sciences
基金
福建省属公益类科研院所基本科研专项(2018R1026-10)
福建省属公益类科研院所基本科研专项(2017R1026-6)
福建省自然科学基金项目(2015J01118)
福建省农业科学院创新团队PI项目(2016PI-40)
关键词
花椰菜
转录组
测序
基因分析
功能注释
Brassica oleracea L. var. botrytis L.
transcriptome,sequencing
gene analysis
function annotation
