摘要
为探究酪氨酸残基化学修饰蚕丝丝素蛋白的反应规律,实现可控化学修饰,以修饰后蚕丝丝素的颜色深度K/S值为衡量指标,分析了碱预处理、重氮盐组分用量、时间、p H值等工艺参数对邻硝基苯胺重氮盐修饰酪氨酸残基的影响规律;研究了重氮盐对酪氨酸残基偶合修饰的吸附过程、吸附等温线类型。结果表明:碱预处理可降低蚕丝丝素的Zeta电位值,同时增加酪氨酸残基结构中酚羟基邻位碳的电子云密度,二者共同促进了蚕丝丝素蛋白对重氮盐的吸附,提高了偶合修饰反应速率;在重氮盐与酪氨酸的量比为2∶3,p H值为6.8、温度为5℃条件下偶合修饰反应45 min,蚕丝丝素纤维可获得较高的K/S值;酪氨酸残基偶合修饰反应吸附等温线符合Langmuir模型。
In order to explore the regulation rules of chemical modification of tyrosine residues to realize the controlled chemical modification of silk fibroin,the effects of processing parameters including alkaline pretreatment,the dosage of diazonium salt,time and pH value on the modification of o-nitroaniline diazonium salt to tyrosine residues were analyzed,using the color depth K/S value of modified silk fibroin as the index.Meanwhile,the adsorption process and type of adsorption isotherm of coupling modification of diazonium salt to tyrosine residues were also studied in the experiments.Results show that the Zeta potential value of silk fibroin is reduced by alkali pretreatment,while the electron cloud density of the phenolic hydroxyl ortho carbon of tyrosine residues is increased,both of which promote the adsorption of silk fibroin fibers on diazonium salt and increase the rate of coupling modification reaction.The silk fibroin fibers are modified in a bath with n(diazonium salt)∶n(tyrosine)=2∶3 at pH of 6.8 at 5℃for 45 min,and then a higher K/S value is obtained.Moreover,the adsorption isotherm of tyrosine residue coupling reaction conforms to the Langmuir model.
作者
杨海伟
王宗乾
徐晶晶
王明荣
王邓峰
YANG Haiwei;WANG Zongqian;XU Jingjing;WANG Mingrong;WANG Dengfeng(School of Textile and Garment,Anhui Polytechnic University,Wuhu,Anhui 241000,China)
出处
《纺织学报》
EI
CAS
CSCD
北大核心
2018年第9期102-108,共7页
Journal of Textile Research
基金
国家自然科学基金项目(51503002)
安徽省自然科学基金项目(1608085QB43)
安徽省高校自然科学研究重点项目(KJ2016A796)
安徽工程大学中青年拔尖人才项目(2016BJRC007)
