摘要
背景:"手风琴"技术治疗骨折不愈合在临床少量应用后效果显著,但还需要进一步的基础研究作为支持。目的:验证"手风琴"技术可治疗新西兰大白兔骨不愈合。方法:建立新西兰大白兔单侧股骨干缺损型非感染性骨折不愈合模型,造模成功后将新西兰大白兔随机分为3组,实验组大白兔应用"手风琴"技术:用外固定架以0.5 mm/d的速度持续加压至4周,第5,6周按照0.5mm/d的速度对骨折断端进行持续牵开,牵开结束后仍以0.5mm/d的速度进行持续加压4周;对照组对骨折断端复位后只进行加压;对空白对照组实验动物不愈合骨折断端进行复位并确保骨折远近端接触,但不进行任何牵张处理,既不加压,也不牵开。然后分别于2,4,6,8,12周分别对兔进行拍X射线片、拍片后对骨折断端取标本,进行苏木精-伊红染色并进行组织学观察。结果与结论:(1)实验组X射线检查提示骨折愈合,而对照组及空白组仍骨折不愈合(P <0.01);(2)组织学检查示实验组骨折不愈合部位可见明显由骨折不愈合状态向愈合状态的转变,最后骨折愈合,对照组及空白对照组未见明显骨折愈合变化;(3)结果表明,"手风琴"技术可简单、微创及高效地治愈骨折不愈合。
BACKGROUND:“Accordion”technique achieves satisfactory outcomes in the treatment of nonunion,but there is a lack of basic research.OBJECTIVE:To verify that the“accordion”technique is effective for nonunion in New Zealand white rabbits.METHODS:The model of unilateral femoral fracture with noninfectious nonunion was created in the New Zealand white rabbits.The model rabbits were then divided into three groups:experimental group:treated with“accordion”technique(4 weeks of pressure with external fixator at a speed of 0.5 mm/d,then 2 weeks of retraction at the same speed,and finally 4 weeks of pressure);control group:external fixation and pressure;blank control group:only external fixation.The rabbits underwent X-ray examination at 2,4,6,8 and 12 weeks.The fracture specimens were taken for hematoxylin-eosin staining and histological observation.RESULTS AND CONCLUSION:The X-ray examination of the experimental group suggested that the fracture was healed,while the control group and the blank control group still did not heal(P<0.01).Histological examination showed that the fracture stump of the experimental group changed from the nonunion state to the healing state,and the blank control and control groups showed no obvious changes.Thus,“accordion”technology is a simple,minimally invasive and efficient treatment method for nonunion.
作者
冯震
张猛
吴薇娜
Feng Zhen;Zhang Meng;Wu Wei-na(Department of Traumatic Orthopedics,Affiliated Hospital of Chengde Medical University,Chengde 067000,Hebei Province,China;Department of Emergency,Affiliated Hospital of Chengde Medical University,Chengde 067000,Hebei Province, China;Department of Radiology,Affiliated Hospital of Chengde Medical University,Chengde 067000,Hebei Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2018年第32期5175-5179,共5页
Chinese Journal of Tissue Engineering Research
