摘要
目的建立双贝胶囊的质量标准。方法采用薄层色谱法对双贝胶囊中土贝母、浙贝母、连翘进行定性鉴别;采用高效液相色谱法对双贝胶囊中土贝母苷甲进行定量分析。结果薄层色谱鉴别分离效果好,专属性强;土贝母苷甲含量在0.182~0.912 mg·m L-1范围内线性关系良好,r=0.9997;平均加样回收率99.31%,RSD 0.49%。结论该方法简单、准确、专属性强、重复性好,可有效控制双贝胶囊的质量。
Objective To establish a quality standard of Shuangbei Capsules.Methods Fritillaria thunbergii,Fritillaria thunbergii Miq,Forsythia in Shuangbei capsules were identified by thin-layer chromatography(TLC).The content of tubeimoside I was determined by high performance liquid chromatography(HPLC).Results The characteristic identification by TLC was distinct and specific.There was a good liner relationship with contents of tubeimosideⅠin the ranges of 0.182-0.912 mg·mL-1 with good correlation coefficient r=0.9997.The average recovery was 99.31%and RSD was 0.49%.Conclusion The established methods are simple,accurate,exclusive,reproducible and suitable for the quality control of Shuangbei capsules.
作者
权小英
马善波
党碧艳
缪珊
QUAN Xiaoying;MA Shanbo;DANG Biyan;MIAO Shan(The Bei Yuan-men Street of West Street Community Health Service Center in Xi'an Lianhu District,Xi'an 710000,China;Department of Pharmacy,Xijing Hospital the Fourth Military Medical University,Xi'an 710032,China;Institute of Materia Medica,the Fourth Military Medical University,Xi'an 710032,China)
出处
《医药导报》
CAS
北大核心
2018年第9期1111-1114,共4页
Herald of Medicine
关键词
双贝胶囊
土贝母苷甲
质量标准
色谱
薄层
色谱法
高效液相色谱
Shuangbei capsules
Tubeimoside I
Quality control standard
Chromatography,thin-layer
Chromatography,high performance liquid
