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柱前衍生化HPLC法建立苦豆子多糖特征图谱的研究 被引量:1

Study on establishing specific chromatogram of Sophora alopecuroides L. polysaccharides by pre-column derivation HPLC method
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摘要 [目的]建立苦豆子多糖特征图谱。[方法]采用1-苯基-3-甲基本5-吡唑酮(PMP)柱前衍生化高效液相色谱(HPLC)法对苦豆子多糖进行单糖组成分析。[结果]苦豆子多糖特征图谱由7个共有峰组成,为甘露糖、葡萄糖、鼠李糖、半乳糖、木糖、阿拉伯糖以及1个未鉴定出的单糖。[结论]柱前衍生化HPLC法建立多糖的特征图谱操作简便、准确、重复性良好,为苦豆子多糖的质量评价提供了依据。 [Objective]To establish specific chromatogram of Sophora alopecuroides L.polysaccharides.[Methods]The monosaccharide composition of Sophora alopecuroides L.polysaccharides was determined by pre-column derivation HPLC method.[Results]There were 7 peaks from the fingerprint which were mannose,glucose,rhamnose,galactose,xylose arabinose and an unidentifiable monosaccharide respectively.[Conclusion]The pre-column derivation HPLC method establishing specific chromatogram is a simple,accurate and reproducible.And the study can provide evidence for quality evaluation of Sophora alopecuroides L.polysaccharides.
作者 张岩 赵振宇 王苹 张桂贤 傅予 刘庆焕 陶遵威 ZHANG Yan;ZHAO Zhen-yu;WANG Ping;ZHANG Gui-xian;FU Yu;LIU Qing-Huan;TAO Zun-wei(Tianjin Institute of Medical and Pharmaceutical Sciences,Tianjin 300020,China;Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital,Tianjin 300120,China;Tasly Holding Group,Tianjin 300410,China)
出处 《天津中医药》 CAS 2017年第9期634-637,共4页 Tianjin Journal of Traditional Chinese Medicine
基金 天津市科技计划项目(12ZCDZSY19200)
关键词 苦豆子多糖 柱前衍生化 HPLC 特征图谱 单糖 Sophora alopecuroides L.polysaccharides pre-column derivation HPLC specific chromatogram monosaccharide
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