摘要
为了建立一种快速、准确测定大蒜多糖含量方法,本研究采用蒽酮-硫酸法测定大蒜多糖含量并对其测定条件进行优化。结果表明:蒽酮-硫酸法测定大蒜多糖含量的精密度实验RSD值为2.10%、平均加样回收率为113%、RSD值为1.17%(n=3)。用本方法测定大蒜籽中多糖含量为31.46%(W/W)。本方法快速、准确、重复性好,完全能满足常规分析要求。
Anthrone-sulfuric method was used to determine content of polysaccharide in garlic seed and the conditions ofcolour reaction of anthrone-sulfuric method were optimized. The experimental results showed that the RSD in accuracy experiment is 2.10%, and the average recovery rate is 113% with RSD=1. 17% （n=3）.The method is rapid, accurate, sensitive and suitable for general analysis.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第9期499-502,共4页
Food Science
基金
国家农业科技成果转化资金资助项目(03EFN213600142)
江西省科技支撑计划项目(赣科发计字[2006]38号)
关键词
大蒜多糖
葸酮
硫酸法
测定
garlic polysaccharide
anthrone-sulfuricmethod
determination
