摘要
采用水提醇沉法从褐蘑菇中提取水溶性多糖,通过苯酚-硫酸比色法在490nm波长处测定多糖含量。在5.00—40.00μg/mL范围内,葡萄糖质量浓度与吸光度呈良好线性关系,相关系数r=0.9981,回收率在97.82%—103.09%之间,相对标准偏差RSD为2.24%(n=5),褐蘑菇多糖含量为3.01%。方法操作简单、显色稳定、准确度高,可作为褐蘑菇多糖工业化生产的常规检测方法。
Polysaccharide from portobello mushroom was extracted with hot water-ethanol method,and was determined at room temperature by phenol-sulphuric acid spectrophotometry 490nm.The standard curve was liner in the concentration range of 5.00—40.00μg/mL with correlation coefficient of r=0.9981.The good recovery is 97.82%—103.09% with relative standard deviation of 2.24%.The contents of polysaccharide from portobello mushroom was 3.01%.This methods have advantages of simple operation,good stability and high accuracy,which can be used as the conventional testing methods of polysaccharide from portobello mushroom in industrial production.
出处
《光谱实验室》
CAS
CSCD
北大核心
2009年第1期43-46,共4页
Chinese Journal of Spectroscopy Laboratory
关键词
褐蘑菇
多糖
提取
分光光度法
Portobello Mushroom,Polysaccharide,Extraction,Spectrophotometry.
