摘要
以蛇菰为材料,采用水提醇沉法提取浓缩后获得蛇菰多糖,以改良苯酚—硫酸比色法测定其多糖量.研究表明,蛇菰中多糖的质量分数为0.38%;加样回收率为100.73%,RSD为1.61%.因此,水提醇沉法可作为蛇菰中多糖的提取方法,改良苯酚—硫酸比色法可以作为蛇菰中多糖的定量测定方法.
In order to determine the content of polysaccharide and confirm the polysaccharide extracting method in Balanophora involucrate Hook.f..Extracting polysaccharides from Balanophora involucrate Hook.f.by the process that was water-decocting followed by alcoholic washing for precipitation.The result showed that the content of polysaccharides was determined by modified phenol-sulphate colorimetry.The contents of polysaccharides extracted in Balanophora involucrate Hook.f.were 0.38%.Recoverieswere 100.73% and relative standard deviation（RSD） are 1.61%.Therefore,the method of water-decocting followed by alcoholic washing can be used as the polysaccharides extracting,and the modified phenol-sulphate colorimetry can be used as determination of the content and the quality control for preparations of polysaccharides in Balanophora involucrate Hook.f.
出处
《昆明学院学报》
2010年第3期44-46,共3页
Journal of Kunming University
关键词
蛇菰
多糖
提取
水提醇沉法
苯酚—硫酸比色法
Balanophore involucrata Hook.f.
polysaccharide
extract
water extracting-alcohol precipitating
phenol-sulphate colorimetry
