摘要
目的分析IL10RA基因功能缺陷所致的极早发型炎症性肠病(VEO-IBD)患儿的肠道菌群特征。方法以复旦大学附属儿科医院消化科病房及门诊为横断面调查现场,纳入IL10RA基因功能缺陷患儿(IL10RA组)、未检测到基因功能缺陷的类似症状患儿(症状组)及年龄匹配的健康儿童(健康组),采用16S rRNA基因测序方法检测3组儿童粪便菌群组成及多样性。使用生物信息学软件对数据去杂,按97%相似性进行OTU聚类后计算多样性指数,并行物种差异判别分析。结果 (1)共纳入IL10RA组17例,症状组15例及健康组22人,收集54份粪便标本。(2)健康组、症状组及IL10RA组平均Shannon多样性指数值分别为1.86±0.53、1.43±0.55及1.11±0.87。(3)健康组厚壁菌门的菌属比例均衡,放线菌门中双歧杆菌属比例97.8%;症状组及IL10RA组中,链球菌属及肠球菌属的平均丰度之和分别占厚壁菌门的61.8%及63.7%。IL10RA组放线菌门中的双歧杆菌属比例低于症状组,罗氏菌属比例高于症状组。(4)17种菌属在IL10RA组及健康组差异有统计学意义(P<0.05),以丰度降低为主。结论 IL10RA基因功能缺陷致VEO-IBD患儿存在肠道菌群紊乱,表现为肠道菌群多样性降低且组内变异度大、菌群分类学结构失衡及肠道共生菌相对丰度降低。
Objective To characterize the microbiome in children with IL10RA deficiency who developed inflammatory bowel disease(IBD)at a very young age.Methods Fecal samples were collected from three groups of children.IL10RA group included very early onset inflammatory bowel disease(VEO-IBD)patients with identified IL10RA deficiency.Symptomatic patients(SP)consisted of patients that presented with signs and symptoms suggestive of VEO-IBD but without gene mutation.And healthy controls(HC)were age-matched volunteers lacking evidence of disease.Microbial DNA was extracted from the fecal samples.All analysis was based on the 16S rRNA gene sequencing data.Results We enrolled 17 IL10RA-deficient patients,15 symptomatic patients,and 22 age-matched healthy children.We observed significantly reduced diversity in IL10RA at both the phylum and ordinal level of taxonomic classification.The Shannon index values for HC,SP,and IL10RA group were 2.37±0.57,1.79±0.70,and 1.36±1.03 respectively.Accordingly,average Simpson index values were 0.19±0.11,0.32±0.20,and 0.48±0.31 respectively.A balanced proportion of genera of Firmicutes and Bifidobacterium dominant Actinobacteria were observed in HC.But in SP and IL10RA group,Firmicutes were dominant by Enterococcus and Streptococcus.Compared with SP group,the proportion of Bifidobacterium was reduced in IL10RA group,but the proportion of Rothia was increased.Seventeen genera showed significance in comparisons of relative abundances between IL10RA group and the other groups.Interestingly,most of them were negatively associated with disease status.Conclusion Intestinal microbiome in VEO-IBD with IL10RA deficiency had features different from the other groups,such as reduced diversity,large variation of the group,imbalanced structure and decreased abundance of symbionts.
作者
薛爱娟
苗士建
孙桦
仇晓霞
王胜楠
黄瑛
XUE Ai-juan;MIAO Shi-jian;SUN Hua;QIU Xiao-xia;WANG Sheng-nan;HUANG Ying(Department of Gastroenterology,Children's Hospital of Fudan University,Shanghai 201102,China)
出处
《中国循证儿科杂志》
CSCD
北大核心
2018年第3期200-204,共5页
Chinese Journal of Evidence Based Pediatrics
基金
玖久专项基金
关键词
IL10RA
极早发型炎症性肠病
肠道菌群
IL10RA
Very early onset inflammatory bowel disease
Intestinal microbiota
