摘要
探讨研究粪便钙卫蛋白与炎症性肠病(inflammatory bowl disease,IBD)活动性的关系。收集52例北京军区总医院消化内镜中心接受肠镜检查患者的新鲜粪便标本提取钙卫蛋白,其中炎症性肠病患者32例、溃疡性结肠炎24例、克罗恩病8例、正常对照者20例。采用ELISA法定量检测粪便中钙卫蛋白浓度。采用Mayo评分系统判断IBD有否活动,积分≥3为有活动性。结果20例健康者粪便钙卫蛋白浓度为(77.15±160.9)μg/g,32例IBD患者为(646.58±439.44)μg/g。IBD患者与健康者粪便钙卫蛋白浓度比较有显著差别(P<0·001)。以450·3μg/g为临界值时,粪便钙卫蛋白判断IBD是否活动的灵敏度和特异度分别为75%和90%,活动期IBD患者与静止期IBD患者和健康者粪便钙卫蛋白浓度比较均有显著差别(P<0.05)。说明粪便钙卫蛋白检测是一种非侵入性观察指标,为判断IBD活动性具有一定的临床意义。
To investigate the relationship between levels of fecal calprotection in the inflammatory bowl disease （IBD） of activated and inactivated by ELISA quantitative analysis, a total of 52 fresh fecal samples were collected from the patients with IBD and the healthy volunteers, including 24 cases of UC, 8 cases of CD, and 20 healthy volunteers. EK-CAL was used to quantitatively determine the level of fecal calprotection. Mayo active index adoptied to evaluate the activity of IBD. The score equal or higher than 3 considered to be active IBD. The level of fecal calprotection was （77.15 ± 160.9） μg/g in healthy volunteers, （956.13 ±488.36） μg/g in active IBD, （337.06 ± 390.52）μg/g in inactive IBD, respeetively. There was signifieanted differenee between the IBD compared with the healthy volunteers （P 〈 0. 001 ）. When IBD beeame active, the level of feeal ealprotection increased significantly compared with the inaetive IBD and healthy volunteers, respectively （ P 〈 0. 05 ）. When the upper limit was 450. 3 μg,/g,the sensitivity and specificity of fecal ealproteetion in differentiating the active IBD was 75% and 90%, respectively. As a non-invasive method, fecal ealproteetion is the marker for evaluate IBD activity, it has a clinical significance.
出处
《科学技术与工程》
2010年第8期1955-1958,共4页
Science Technology and Engineering
