摘要
【目的】研究刺山柑(Capparis spinosa L.)的化学成分。【方法】运用硅胶柱色谱、凝胶柱色谱(Sephadex LH-20)和反相RP-18柱色谱对乙酸乙酯部位进行分离纯化,然后通过现代波谱学鉴定方法 (1H-NMR,13CNMR,MS),结合文献数据分析确定化合物的结构。【结果】从刺山柑乙酸乙酯部位分离得到12个化合物,依次确定为(R)-2-甲基-2,4-二甲氧基-6H-吡喃-3-酮(1),1H-吲哚-3-乙腈-4-O-β-吡喃型葡萄糖苷(2),3-吲哚甲酸(3),尿嘧啶(4),5-(methoxymethyl)-1H-pyrrole-2-carbaldehyde(5),4-羟基-5-甲基呋喃-3-羧酸(6),苯甲酸(7),水杨酸(8),(2R,4a R,8a R)-3,4,4a,8a-tetrahydro-4a-hydroxy-2,6,7,8a-tetramethyl-2-(4,8,12-trimethyltridecyl)-2Hchromene-5,8-dione(9),α-生育酚(10),异槲皮苷(11),芸香苷(12)。【结论】化合物1,3,5,6,9,10均为首次从刺山柑中分离得到。
[Purpose]The chemical constituents of Capparis spinosa L.were analyzed.[Method]The compounds were isolated by silica column chromatography,Sephadex LH-20 column chromatography and RP-18 reverse-phase column chromatography from the EtOAc extract.The structures were characterized by various spectroscopic(1H-NMR,13C-NMR and MS)and comparison with the data of literatures.[Result]Twelve compounds were isolated from the EtOAc extract,and identified as(R)-2,4-dimethoxy-2-methyl-6H-pyran-3-one(1),capparilosides A(2),indole-3-carboxylic acid(3),uracil(4),5-(methoxymethyl)-1H-pyrrole-2-carbaldehyde(5),4-hydroxy-5-methylfuran-3-carboxylic acid(6),benzoic acid(7),salicylic acid(8),(2R,4aR,8aR)-3,4,4a,8a-Tetrahydro-4a-hydroxy-2,6,7,8a-tetramethyl-2-(4,8,12-trimethyltridecyl)-2H-chromene-5,8-dione(9),α-tocopherol(10),isoquercitrin(11),rutin(12).[Conclusion]Compounds 1,3,5,6,9,10 were isolated from C.spinosa L.for the first time.
作者
何江波
欧阳芬
张燊
王波
句红萍
陶剑
张雪梅
芮丹云
牛燕芬
HE Jiangbo;OUYANG Fen;ZHANG Shen;WANG Bo;JU Hongping;TAO Jian;ZHANG Xuemei;RUI Danyun;NIU Yanfen(Kunming Key Laboratory of Respiratory Disease,Kunming University,Kunming 650214,China;Kunming Arts School,Kunming 650216,China;School of Agriculture,Kunming University,Kunming 650214,China)
出处
《云南农业大学学报（自然科学版）》
CSCD
北大核心
2018年第3期558-562,共5页
Journal of Yunnan Agricultural University:Natural Science
基金
云南省科技厅青年基金项目(2016FD109)
云南省教育厅资助项目(2017ZZX091)。
关键词
刺山柑
提取分离
化学结构
结构鉴定
Capparis spinosa L.
extraction and isolation
chemical constituents
structural identification
