摘要
对患病中华鳖(Pelodiscus sinensis)进行病原分离、鉴定及药敏实验,从患病中华鳖皮肤、肝肾脾重要器官分离纯化病原菌,经理化特性测定及16S r RNA序列分析对其进行鉴定及人工感染试验,并利用K-B及二倍稀释法进行药敏特性分析。结果表明分离株J22是为中华鳖腐皮病病原,其对中华鳖的LD50为3.30×104 CFU/g。J22株理化特性与产吲哚金黄杆菌(Chryseobacterium indologenes)一致,16S r RNA序列与产吲哚金黄杆菌同源性为99%,综合判定J22株是产吲哚金黄杆菌。分离株对新霉素、庆大霉素及阿莫西林等12种抗生素高度敏感,对氟苯尼考及多西环素等抗生素耐药;二氧化氯、漂白粉及高铁酸钾对分离株消毒效果较好。分离菌株J22是中华鳖病原菌,养殖时可选用庆大霉素、新霉素或者阿莫西林内服,配合使用二氧化氯、漂白粉及高铁酸钾等外用进行防控。
This study conducted isolation,identification and drugs sensitivity of pathogen from sick Trionyx sinensis to provide a reference for control and prevention disease for Trionyx sinensis.The pathogenic bacteria were isolated and purified from skin,liver,kidney and spleen of Trionyx sinensis.The identification of the isolated pathogen was analyzed by biochemical reactions and 16S rRNA gene sequence.The artificial infection test was performed with intraperitoneal injection,and drugs susceptibility test was conducted by K-B method and double broth dilution method.The results showed that strain J22 was the pathogen of Trionyx sinensis,and the LD50 was 3.30×104 CFU/g.According to morphological and biochemical characteristics as well as 16S rRNA gene sequence analysis,strain J22 was identified as Chryseobacterium indologenes.Strain J22 was susceptible to neomycin,gentamicin and amoxicillin and other 9 antibiotics but resistant to florfenicol and doxycycline,etc.The chlorine dioxide,bleaching powder and potassium ferrate had great antiseptic effect on the isolated pathogeb.These results indicate that Strain J22-induced disease of Trionyx sinensis can be treat by drugs such as neomycin,gentamicin and amoxicillin for oral administration,and that chlorine dioxide,bleaching powder and potassium ferrate are suitable for external use in farming.
作者
杨移斌
艾晓辉
宋怿
曹海鹏
杨先乐
姚嘉赟
沈锦玉
YANG Yi-Bin;AI Xiao-Hui;SONG Yi;CAO Hai-Peng;YANG Xian-Le;YAO Jia-Yun;SHEN Jin-Yu(Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture,Key Laboratory of Fish Health and Nutrition of Zhejiang Province,Zhejiang Institute of Freshwater Fisheries,Huzhou 313001,China;Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Wuhan 430223,China;Key Laboratory of Control of Quality and Safety for Aquatic Products,Ministry of Agriculture,Beijing 100141,China;Shanghai Ocean University,Shanghai 201306,China)
出处
《水生生物学报》
CAS
CSCD
北大核心
2018年第4期786-793,共8页
Acta Hydrobiologica Sinica
基金
农业部淡水渔业健康养殖重点实验室开放课题(ZJK201510)
公益性行业(农业)科研专项(201503108-CC-1、201203085)
现代农业产业技术体系建设专项(CARS-49)
现代农业人才支撑计划
中国水产科学研究院基本科研业务费(2017HY-ZD 0505和2016 HY-ZD1202)资助。
关键词
产吲哚金黄杆菌
中华鳖
分离鉴定
人工感染
药敏特性
Chryseobacterium indologenes
Trionyx sinensis
Isolation and identification
Artificial infection
Antibiotic sensitivity