摘要
目的了解深圳地区急性上呼吸道感染(acute upper respiratory tract infection,AURTI)住院患儿人偏肺病毒(human metapneumovirus,hMPV)的感染现状及hMPV优势亚型的流行情况。方法收集2015年6月～2016年6月在深圳市龙华区人民医院和福田人民医院因ALRTI住院的患儿鼻咽吸取物或咽拭子标本1720份,同时收集同期健康儿童呼吸道标本6 0份,采用Qiagen One-step RT-PCR法对标本中的hMPV进行筛检,并随机选取6例hMPV阳性标本采用CEQ8800测序仪对扩增产物进行正反测序,采用BLAST软件将测序所得的基因序列与GenBank中的代表株进行序列比对分析。结果 1 720例AURTI住院患儿的鼻咽吸取物或咽拭子标本共检出hMPV阳性标本362例,阳性率为21.05%(362/1720),其中男童阳性率为10.06%(173/1720),女童阳性率为10.99%(189/1720),不同性别患儿阳性率的差异无统计学意义(χ~2=0.917,P>0.05),而健康儿童hMPV全为阴性;<1岁组AURTI住院患儿hMPV感染阳性率为3 1.4 6%,明显高于其他组,差异有统计学意义(χ~2=6.105～11.97 3,P(0.05～0.01);1～3月AURTI住院患儿感染hMPV阳性率最高为4 9.7 4%,明显高于其他月份,差异有统计学意义(χ~2=7.927～1 5.038,P<0.05～0.01);6株hMPV阳性毒株的病毒核苷酸与代表株同源性为91.0%～100.0%;6株hMPV阳性毒株分别属于A2b和B1及B2亚型。结论 hMPV是引起深圳地区AURTI住院患儿感染的主要病原之一,好发于冬春季节和1岁以下儿童,且同时存在A和B两个基因型流行。
Objective To understand acute upper respiratory trace infections(acute upper respiratory tract infection,AURTI)people hospitalized children with partial pulmonary virus(human metapneumovirus,hMPV)infection status and the prevalence of hMPV advantage subtypes in Shenzhen area.Methods From June 2015 to June 2016 in shenzhen longhua district people's hospital and Hutian people's hospital hospitalized Children with AURTI nasopharyngeal drain or swallow test specimens 1 720,at the same time respiratory specimens from 60 healthy children of the same period were collected,Qiagen One-step RT-PCR method for screening of hMPV in the sample was employed,and amplification products randomly selected from 6 cases of hMPV positive specimens were sequenced by CEQ8800 sequencer through forward and reverse primers,BLAST software was used to sequence alignment analysis for sequencing of gene sequences with GenBank representative strains.Results Nasopharyngeal hMPV positive specimens of 362 cases were detected from 1 720 cases of hospitalized children with AURTI drains content or swallow test specimen,positive rate was 21.05%(362/1 720),the boy's positive rate was 10.06%(173/1 720),the girl's positive rate was 10.99%(189/1 720),there was no statistically significant difference between male and female children with positive rate(^2=0.917,P>0.05),and healthy children hMPV were all negative;<1 year old group AURTI hospitalized with an hMPV infection rate was 31.46%,significantly higher than the other group,and the difference was statistically significant(X2=6.105?1 1.973,P<0.05?0.01);1?3 month AURTI hospitalized children with hMPV infection positive rate was 49.74%,significantly higher than the other month,difference was statistically significant between(X2=7.927?15.038,P<0.05?0.01);The virus nucleotide of the six hMPV positive strains was 91.0%?100.0%.6 strains of hMPV positive strains belong to the A2b and B1 and B2 subtype respectively.Conclusion hMPV is one of the main pathogens of infection in children with AURTI in the hospital i
作者
刘爱玲
刘淑燕
朱彩云
郭龙华
刘军
LIU Ai-ling;LIU Shu-yan;LIU Jun;ZHU Caiyun;GUO Longhua
出处
《临床输血与检验》
CAS
2018年第2期150-153,共4页
Journal of Clinical Transfusion and Laboratory Medicine
基金
广东医科大学校院联合科研基金(No.L2016008)资助
关键词
急性上呼吸道感染
患儿
人偏肺病毒
现状
基因分析
Acute upper respiratory tract infection
Children
Human pneumovirus
Status
Genetic analysis
