摘要
目的分析F框/WD-40域蛋白7(FBXW7)及c-Myc蛋白在人胃癌组织中的表达及其与胃癌临床病理特征的相关性。方法回顾性收集102例胃癌临床手术标本,每份标本均取其正常胃黏膜组织(A组)、癌旁组织(B组)与癌组织(C组)。通过免疫组织化学法对三种组织FBXW7及c-Myc蛋白的表达水平进行检测,进而分析其与胃癌临床病理特征的相关性。结果相比A组与B组,C组中的FBXW7蛋白表达水平显著降低(P<0.05),而c-Myc蛋白的表达水平明显升高(P<0.05);而B组中的FBXW7、c-Myc蛋白表达水平均显著低于A组(P<0.05)。FBXW7、c-Myc蛋白的表达水平与患者年龄、性别及肿瘤部位均无显著相关性(P>0.05),但与胃癌分化程度、肿瘤浸润深度、淋巴结转移及TNM分期均显著相关(P<0.01)。结论胃癌患者中FBXW7蛋白表达水平明显降低,而cMyc蛋白表达明显升高,二者表达水平可能与肿瘤分化程度、浸润深度及发生淋巴结转移等存在密切联系,FBXW7与c-Myc可能成为早期诊断与评估胃癌浸润及淋巴结转移的重要生物学指标。
Objective To analyze the expression of F-Box and WD40 domain protein 7(FBXW7)and c-Myc protein in human gastric carcinoma and its correlation with clinicopathological features of gastric carcinoma.Methods The clinical specimens of 102 cases of gastric cancer were collected in our hospital.Each specimen was taken from normal gastric mucosa tissue(group A),paracancerous tissue(group B)and carcinoma tissue(group C).The expression levels of FBXW7 and c-Myc proteins in three tissues were detected by immunohistochemistry,and the correlation between FBXW7,c-Myc and clinicopathological features of gastric cancer was analyzed.Results Compared with the group A and group B,the expression level of FBXW7 protein in the group C decreased significantly(P<0.05),and the expression level of c-Myc protein was significantly increased(P<0.05).The expression level of FBXW7 and c-Myc protein in the B group were significantly lower than those in the group A(P<0.05).The expression levels of FBXW7 and c-Myc protein weren't significantly correlated with the age,gender and tumor location(P>0.05),but they were significantly correlated with the differentiation degree,the depth of tumor invasion,lymph node metastasis and TNM stage(P<0.01).Conclusion The expression levels of FBXW7 decrease significantly in the patients with gastric carcinoma,and the expression levels of c-Myc protein significantly increase,and the expression levels of two protein is closely related with the differentiation degree.The depth of tumor invasion,lymph node metastasis and other indexes,which indicate that FBXW7 and c-Myc may be important biological markers for early diagnosis and assessment of cancer invasion and lymph node metastasis gastric carcinoma.
作者
张伟
马晓龙
艾建中
ZHANG Wei;MA Xiao-long;AI Jian-zhong(Department of General Surgery,Mianyang Central Hospital,Mianyang Sichuan 621099,China)
出处
《临床和实验医学杂志》
2018年第7期751-754,共4页
Journal of Clinical and Experimental Medicine