摘要
目的建立同时测定解郁安神颗粒中甘草苷、甘草酸含量的高效液相色谱(HPLC)法。方法色谱柱为Welch Ultimate XD-C_18柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.05%磷酸溶液,梯度洗脱,流速为1.0 mL/min,柱温为35℃,检测波长为237 nm。结果甘草苷、甘草酸质量浓度分别在13.68~342.00μg(r=0.999 2)及23.48~587.00μg(r=0.996 8)范围内与峰面积线性关系良好,平均加样回收率分别为94.64%和97.55%(n=6)。各组分基线分离,阴性样品无干扰。结论该方法可作为解郁安神颗粒多组分同时分析和质量控制的方法。
Objective To establish an HPLC method for simultaneous determination of licorice glucoside and glycyrrhizic acid in Jieyu Anshen Granules.Methods Weich Ultimate XD-C18 column(250 mm×4.6 mm,5μm)was adopted,the mobile phase was acetonitrile-0.05%phosphoric acid with gradient elution,the flow rate was 1.0 mL/min,the column temperature was 35℃,and the detection wavelength was 237 nm.Results The licorice glucoside and glycyrrhizic acid had good linear relationship in the ranges of 13.68-342.00μg(r=0.99),23.48-587.00μg(r=0.99),the average recoveries were 94.64%and 97.55%,espectively.The baseline was separated in each group,and the negative sample had no interference.Conclusion The method can be used for the simultaneous analysis and the quality control of Jieyu Anshen Granules.
作者
樊宝娟
黄艳
罗定强
吴芳
杨亚丽
戴涌
FAN Baojuan;HUANG Yan;LUO Dingqiang;WU Fang;Yang Yali;DAI Yong(Shaanxi Institute for Food and Drug Control,Shanxi Xi'an 710061,China)
出处
《中国药业》
CAS
2018年第4期13-15,共3页
China Pharmaceuticals
基金
2016年国家评价性抽验课题
陕西省中医药管理局中医药科研课题[ZY24]
关键词
高效液相色谱法
解郁安神颗粒
甘草苷
甘草酸
含量测定
HPLC
Jieyu Anshen Granules
licorice glucoside
glycyrrhizic acid
content determination
