摘要
通过对史氏鲟鱼软骨进行提取工艺的单因素试验,以及三因素三水平的正交试验,结果表明:最佳提取工艺条件为酶解温度40℃、酶用量0.5%、酶解pH值8.2,其酶解提取的硫酸软骨素浓度为59.07%。利用该提取物对小白鼠背部伤口进行创面愈合效果试验,结果表明:给药后20d,创口愈合良好,疤痕不明显,再生皮肤光滑如常且有毛发再生。
Extraction technology for Acipenser schrenckii cartilage was optimized by single-factor experiments and L9(33)orthogonal experiment.The results showed that the optimum extraction conditions were:enzyme hydrolysis temperature was at 40℃,enzyme usage was 0.5%,pH value for enzyme hydrolysis was at 8.2,and the concentration of chondroitin sulfate extracted was 59.07%.Promotion of the extraction on healing the wounded back of white rat was studied,it was found that when using the solution of Acipenser schrenckii cartilage extraction for 20 days,the wounds on the white rats were healed with no obvious scars,and the regenerated skin was smooth as when original with regenerated hair.
作者
饶秋华
罗土炎
曹治云
罗钦
李弈君
王少珍
张志灯
RAO Qiu-hua;LUO Tu-yan;CAO Zhi-yun;LUO Qin;LI Yi-jun;WANG Shao-zhen;ZHANG Zhi-deng(Institute of Quality Standards and Testing Technology,Fujian Academy of Agricultural Sciences/Fujian Key Laboratory of Precision Measurement for Agriculture,Fujian Province 350003;College of Integrated Chinese and Western Medicine,Fujian University of Traditional Chinese Medicine;Inspection and Quarantine Technique Centre of Fujian Entry-exit Inspection and Quarantine Bureau)
出处
《福建农业科技》
2017年第10期1-5,共5页
Fujian Agricultural Science and Technology
基金
福建省发展改革委员会战略性新兴产业发展专项(闽发改高技[2013]266号)
福建省科技计划项目--省属公益类科研院所基本科研专项(2016R1024-2)
福建省农业科学院院管B类项目(B2012-02)
关键词
史氏鲟鱼
硫酸软骨素
小白鼠
创面愈合
Acipenser schrenckii
chondroitin sulfate
white rat
wound healing
