摘要
为研究人白细胞ＤＲ抗原（ｈｕｍａｎｌｅｕｋｏｃｙｔｅａｎｔｉｇｅｎ－ＤＲ，ＨＬＡＤＲ－ＤＲ）和硫酸软骨素蛋白聚糖（ｃｈｏｎｄｒｏｉｔｉｎｓｕｌｆａｔｅｐｒｏｔｅｏｇｌｙｃａｎ，ＣＳ－ＰＧ）在人冠状动脉中的表达，将１３例年轻人（１５岁～３９岁）的冠状动脉（左旋支或左前降支）用福尔马林固定后，进行石蜡包理，其中６例为对照组（正常组），７例为病变组（脂纹组）。连续切片后，应用微波炉对两组各例进行ＨＬＡ－ＤＲ和ＣＳ－ＰＧ免疫组织化学染色。切片经图象分析仪定量后，所得结果显示，病变组内膜ＨＬＡ－ＤＲ阳性细胞数密度和ＣＳ－ＰＧ阳性细胞面密度都分别明显高于对照组（Ｐ＜０．０１），并且对照组和病变组内膜ＨＬＡ－ＤＲ数密度和ＣＳ－ＰＧ面密度都呈显著正相关（Ｐ＜０．０１）。从脂纹组中任取一例进行ＣＤ６８、增殖细胞核抗原的免疾组织化学染色，经镜下观察发现，ＣＤ６８阳性细胞同时也是ＨＬＡ－ＤＲ阳性；ＨＬＡ－ＤＲ阳性细胞大部分是增殖细胞核抗原阳性。提示冠状动脉粥样硬化早期病变中ＨＬＡＤＲ表达与泡沫细胞的形成、平滑肌细胞的增殖、ＳＣＰＧ增多有密切关系。
In order to study expression of human leukocyte antigen-DR (HLA-DR) and chondroitin sulfate proteoglycan (CS-PG) in human coronary artery , 13 coronary arieries including normal group (n=6) and lesion group (n=7),which were fixed with formalin, embedded in paraffin and slided successively (4μm ), were stained with HLA-DR and CS-PG microwave immunohistochemistry. Then the slides were quantitated with image analyzer. Results indicated that the numerical density of HLA-DR positive cells and the area density of CS-PG in the intima of lesion group were evidently higher than that of normal group, and were significantly correlative with that of normal group. One of lesion group was stained with proliferative cell nuclear antigen (PCNA) and CD68. Similar localization of CD68 and HLA-DR was found in intima. Menatime, positive cells of HLA-DR mostly were positive ones of PCNA. These results indicated that expression HLADR in earlier As lesion was closely related with formation of foam cells, proliferation of SMC and increase of CS-PG.
出处
《中国动脉硬化杂志》
CAS
CSCD
1995年第3期232-235,共4页
Chinese Journal of Arteriosclerosis
基金
国家八.五攻关课题
关键词
动脉粥样硬化
HLA-DR抗原
CS-PG
免疫组织化学
Earlier atherosclerotic lesion
Human leukocyte antigen
Proliferative cell nuclear antigen
Microwave immunohistochemistry
Chondroitin sulfate proteoglycan
