摘要
目的:探讨AB-8大孔树脂对山葡萄籽多酚提取物的纯化工艺参数,确定最佳纯化工艺条件,为山葡萄籽的进一步开发利用提供技术参考。方法:采用酿酒后的山葡萄籽提取物为原料,检测山葡萄籽多酚浓度并计算多酚的吸附率和解吸率;采用AB-8大孔树脂纯化山葡萄籽多酚提取物,通过吸附-解吸性能考察优选山葡萄籽粗提物的最佳纯化工艺。对比山葡萄籽提取物纯化前后的多酚浓度,验证纯化工艺效果。结果:AB-8大孔吸附树脂对山葡萄籽多酚粗提物的吸附率和解吸率分别为90.48%和71.42%,吸附性能和解吸性能良好。确定最佳吸附条件为,样品溶液浓度10g·L-1,上样流速3mL·min-1;最佳解吸条件为,洗脱液浓度60%,洗脱流速2mL·min-1,洗脱体积为2倍柱体积。在该工艺条件下,纯化后山葡萄籽多酚浓度从35.02%提高到88.80%。结论:AB-8大孔树脂纯化山葡萄籽多酚效果良好,值得推广应用。
Objective:To explore the purification technology parameters for polyphenols from vitis amurensis seed extract with AB8macroporous resin and to establish the optimum purification process conditions,and to provide technical reference for further development and utilization of vitis amurensis seeds.Methods:The vitis amurensis seeds were regarded as raw material,the concentration of vitis amurensis seed polyphenols was detected,and the adsorption rate and desorption rate of polyphenols were calculated.The AB8macroporous resin was used to purify the polyphenols from vitis amurensis seed extract,and the purification technology parameters of vitis amurensis seed polyphenols were optimized by absorption desorption experiment.The concentrations of polyphenol from vitis amurensis seed extract were.compared between before and after purification,and the effect of the purification process was verified.Results:The AB8macroporous resin had good absorption and desorption properties to crude the extract of vitis amurensis seed polyphenols,and the adsorption rate and desorption rate were90.48%and71.42%.The optimum adsorption conditions and desorption conditions were as follows:the sample concentration was10g·L-1,the sample flow rate was3mL·min-1,the eluting concentration was60%,the elution velocity was2mL·min-1,and the eluting volume was2fold column volume.Under this condition,the concentration of polyphenols from vitis amurensis seed extract after purification was increased from35.02%to88.8%.Conclusion:AB8macroporous resin has better purification effect,and it is worthy of popularization and application.
作者
季红
贾荣
郭鑫
JI Hong;JIA Rong;GUO Xing(Department of Pharmacy Service,Chain Japan Union Hospital,Jinlin University,Changchun 130033,China;Online Centralized Purchasement Service Management Center for Drug and Medical Consumables of Medical Institution,Baotou City,Inner Mongolia Autonomous Region,Baotou 014010, China)
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2017年第6期1272-1277,共6页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅科研基金资助课题(200302-05)
关键词
山葡萄籽
多酚
大孔树脂
纯化
vitis amurensis seeds
polyphenols
macroporous resin
purification
