摘要
采用Folin-Ciocalteu比色法研究了橄榄多酚的定量测定方法.结果表明:橄榄多酚提取液在加入Folin-Ciocalteu试剂1.5 mL、10%Na2CO3 6 mL、反应时间2 h、温度30 ℃的条件下进行显色,并于765 nm处测定其吸光值,多酚质量浓度在7～70 mg/L范围内与吸光值有良好的线性关系,根据此线性回归方程进行橄榄多酚的定量测定,该方法简单方便、稳定性好、准确度高.
A quantitative determination method of polyphenol from Canarium album (Lour.) Rauesch. was studied by Folin-Ciocalteu colorimetry. The results showed that polyphenol content of C. album could be well calculated according to their colorime- tric absorption at 765nm by applying Folin-Ciocalteu reagent 1.5mL and 10% Na_2CO_3 6mL , 2h at 30℃ for colorimetric reaction, and the linear range of standard curve was 7-[KG-*6] 70mg/L . The method established was very convenient, stable and accurate.
出处
《林产化学与工业》
EI
CAS
CSCD
2006年第4期15-18,共4页
Chemistry and Industry of Forest Products
基金
国家科技部农业科技成果转化资金资助项目(03EFN217100327)
关键词
橄榄
多酚
比色法
Canarium album (Lour.) Rauesch.
polyphenol
colorimetry
