摘要
目的:在大鼠心肌线粒体(Mt)自由基损伤的体外实验中观察山葡萄多酚(PVAR)对心肌线粒体氧化损伤的保护作用,为治疗心肌缺血再灌注损伤提供实验依据。方法:实验分5组,正常线粒体组、诱导损伤线粒体组和预先加25、50、100mg·L-1PVAR再诱导损伤线粒体组;利用Fe2+/维生素C(VitC)系统产生氧自由基,体外对Wistar大鼠心肌Mt诱导损伤,观察各组Mt心磷脂和MDA含量、膜流动性、ATPase活性和肿胀度的变化。结果:与损伤组比较,三种剂量PVAR组心磷脂含量明显增高(P<0.05),MDA明显减少(P<0.01),膜流动性显著增大(P<0.01),ATPase活性增高(P<0.05),100mg·L-1PVAR组线粒体肿胀度与正常组比较差异无显著性。结论:氧自由基引起Mt损伤;PVAR对氧自由基引起的大鼠心肌Mt损伤具有保护作用,保护机制可能是直接减少氧自由基的生成。
Objective To study the protective effects of the polyphenols of vitis amurensis Rupr （PVAR） on rat heart mitochondria injury induced by oxygen radicals. Methods Experiment was designed into 5 groups： normal group, injury group and 25, 50, 100 mg· L^-1 PVAR＋injury group. In experiment of rat heart mitochondria injury in vitro, vitamin C and FeSO4 played as an injury agent, PVAR played as a protective agent. The cardiolipin and MDA level of the mitochondria were determined. The membrane fluidity, ATPase activity and swelling of the mitochondria were examined. Results Compared with injury group, the cardiolipin was increased （P〈0.05）, the MDA was decreased （P〈0.05）, the membrane fluidity and the ATPase activitiy were increased （P〈0.01） in 25, 50, 100 mg·L^-1 PVAR groups. There was no significant difference of swelling of injured mitochondria between PVAR （ 100 mg ·L^-1） group and control group. Conclusion Oxygen radicals can result in severe mitochondria injuried. The PVAR can protect the injuried rat heart mitochondria by antagonizing the free radicals.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2008年第1期117-119,共3页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅基金资助课题(200302-05)
关键词
山葡萄
多酚
线粒体
膜流动性
vitis amurensis Rupr
polyphenols
mitochondria
membrane fluidity
