摘要
文章以尾叶桉(Eucalypt urophylla)优良新无性系的无菌组培苗为材料,MS培养基为基本培养基,研究6-BA、NAA不同质量浓度配比对尾叶桉无菌幼苗丛芽增殖率,以及生根粉种类、质量浓度、不同IBA和NAA配比对不同尾叶桉无性系生根率的影响。试验结果表明,当6-BA质量浓度为0.01 mg/L,丛芽增殖率在NAA质量浓度为0.01、0.1 mg/L时递增;而NAA质量浓度为1、5 mg/L时,则有利于生根;筛选出适宜尾叶桉优良无性系ZQUA10的增殖培养基为MS+0.01 mg/L 6-BA+0.01 mg/L NAA+30 g/L蔗糖+6 g/L卡拉胶,增殖系数达到4.7;筛选出适宜尾叶桉优良无性系ZQUA13的生根培养基为1/2MS+0.5mg/L ABT1+30 g/L蔗糖+6 g/L卡拉胶,生根率达到57.5%;筛选出适宜尾叶桉优良无性系ZQUB58的生根培养基为1/2MS+1 mg/L IBA+0.5 mg/L NAA+30 g/L蔗糖+6 g/L卡拉胶,生根率达到100%。
The paper studied the effects of different6-BA and NAA concentrations on aseptic seedling clusterbuds proliferation rate as well as the effects of different rooting powder and concentration on rooting rate ofEucalyptus urophylla tissue culture seedling with MS medium as basic medium.The study aimed to provid theoreticalguidance for the establishment of efficient tissue culture and rapid propagation system and accelerat theefficient utilization of fine varieties and factory production of E.urophylla.The results showed:With the treatment0.01mg/L6-BA and0.01、0.1mg/L NAA,the cluster buds proliferation rate reached the highest;it was beneficialto the growing of roots when NAA concentration was1、5mg/L;the proper medium for clonal variety of E.urophylla(ZQUA10)was MS+0.01mg/L6-BA+0.01mg/L NAA+30g/L sucrose+6g/L carrageenan,the proliferationcoefficient reached4.7.The proper medium for clonal variety of E.urophylla(ZQUA13)was1/2MS+0.5mg/L ABT1+30g/L sucrose+6g/L carrageenan,the rooting rate reached57.5%.The proper medium forclonal variety of E.urophylla(ZQUB58)was1/2MS+1mg/L IBA+0.5mg/L NAA+30g/L sucrose+6g/L carrageenanand the rooting rate reached100%.
作者
廖焕琴
黄晓玲
潘文
张卫华
LIAO Huanqin;HUANG Xiaoling;PAN Wen;ZHANG Weihua(Guangdong Academy of Forestry/ Guangdong Provincial Key Laboratory of Silviculture, Protection and Utilization, Guangzhou,Guangdong 510520,China;Zhongkai University of Agriculture and Engineering, Guangzhou,Guangdong 510225, China)
出处
《林业与环境科学》
2017年第2期36-41,共6页
Forestry and Environmental Science
基金
广东省省级科技计划项目"广东主要商品林种业关键技术研究"(2015B070701009)
关键词
尾叶桉
组织培养
再生体系
Eucalyptus urophylla
tissue culture
regeneration system
