摘要
目的：建立测定养阴合剂中黄芩苷含量的方法。方法：采用高效液相色谱法。色谱柱为SunFireC18，流动相为甲醇-水-磷酸（43∶57∶0.2，V/V/V），流速为1.0ml/min，检测波长为280nm，柱温为30℃，进样量为10μl。结果：黄芩苷的检测进样量线性范围为0.05575～1.115μg（r＝0.9999）；精密度、稳定性、重复性试验的RSD＜2％；加样回收率为95.39％～98.44％（RSD＝1.19％，n＝6）。结论：该方法简便、快速，结果准确，适用于养阴合剂中黄芩苷的含量测定。
OBJECTIVE:To establish a method for the content determination of baicalin in Yangyin mixture. METHODS:HPLC was performed on the column of SunFire C18 with mobile phase of methnol-water-phosphoric acid(43 ∶ 57 ∶ 0.2,V/V/V)at aflow rate of 1.0 ml/min,the detection wavelength was 280 nm, column temperature was 30 ℃,and the volume injection was 10μl. RESULTS:The linear range of baicalin was 0.055 75-1.115 μg(r=0.999 9);RSDs of precision,stability and reproducibilitytest were lower than 2%;recovery was 95.39%-98.44%(RSD=1.19%,n=6). CONCLUSIONS:The method is simple,rapidand accurate,and suitable for the content determination of baicalin in Yangyin mixture.
作者
车坷科
杨帆
邓开英
CHE Keke;YANG Fan;DENG Kaiying(Dept. of Pharmacy,Chongqing Third People’s Hospital,Chongqing 400014,China;Chongqing Institute for Food and Drug Control,Chongqing 401121,China;Chongqing Engineering Center for Pharmaceutical Process and Quality Control,Chongqing 401121,China)
出处
《中国药房》
CAS
北大核心
2016年第24期3410-3412,共3页
China Pharmacy
基金
重庆市卫生计生委医学科研计划项目(No.2015MSXM067)
