摘要
将辣根过氧化物酶注入大鼠小脑后,在脊髓L_1～L_3节段中的脊髓边缘细胞(SBC)得到逆行标记。对标记的SBC的突触联系进行电镜观察,并根据突触前区小泡形状及终扣形态,将这些终扣大致可分为6型,即S、F、Cf、T、G和P型。其中S和F型终扣根据其形态,各自又分为两个亚型,即细长型和圆型。S和F型分别含有球形及扁平型小泡;Cf型终扣含扁形小泡,突触前、后膜无明显特征,在突触后膜下有亚突触间隙;T型终扣含有清亮球形小泡,也可见大颗粒小泡,在突触后膜下可见致密体;G型终扣在突触前区内含有大颗粒小泡;P型是指附着在大S型终扣上的含有多样形小泡的终扣。S型、T型和G型突触后膜均比前膜明显增厚,呈不对称性。有关上述SBC突触终扣来源问题,还需进一步研究。
Following injection of HRP into the cerebellum of the rat, the retrogradely labeled′spinal border cells′ in L_1-L_3 of the spinal cord were examined electron microscopically for understanding the synaptology. Six types of terminal boutons (S, F, Cf, T, G and P types) which terminated on the surface of cell bodies or dendrites of the spinal border cells were recognized. The S- and F-boutons contain spherical and elliptical vesicles respectively. These two types of boutons had relatively broad area contacting with the surface of either cell bodies or dendrites. They could be derided into two sub-types respectively. One was the elonagted giant bouton and the other was the round. Cf-type contain flattened vesicles and it make membraneous′contact′ lacking in specialized pre-and postsynaptic membrane thickenings. T-type contain spherical and large granular vesicles, and dense body was seen beneath the post synaptic membrane. G-type boutons contain large granular vesicles in area of presynaptic. P-type boutons form synapses upon the large S-type boutons and contain pleomorphic vesicles. The postsynaptic membrane of S-, T- and G-types is apparently thicker than the presynaptic membrane and showed to be asymmetrical. Further study is necessary with regard to the sources of different terminal boutons contacting with different portions of the spinal border cells.
出处
《解剖学报》
CAS
CSCD
北大核心
1991年第4期371-376,T005,T006,共8页
Acta Anatomica Sinica
关键词
突触
HRP
电镜
Synaptology
Spinal border cells
Electron microscopy
HRP
Rat
