摘要
目的 观察模拟失重对大鼠脊髓小脑中间区Ⅲ、Ⅳ小叶皮层和小脑间位核内γ-氨基丁酸(gaba)免疫反应性的影响.方法 将大鼠按随机配对原则分为2组:模拟失重14 d组及正常对照组,每组各10只.采用大鼠尾部悬吊法建立模拟失重模型,用免疫组织化学技术观察大鼠脊髓小脑中间区Ⅲ、Ⅳ小叶皮层和小脑间位核内gaba免疫阳性神经元数量.结果 正常大鼠脊髓小脑中间区Ⅲ、Ⅳ小叶皮层的3个细胞层内以及问位核均有gaba阳性神经元存在.模拟失重14 d后大鼠脊髓小脑中间区Ⅲ、Ⅳ小叶分子层内gaba阳性神经元数量明显减少(t=3.488,p<0.05),而浦肯野细胞层、颗粒层及间位核内gaba阳性神经元数量没有明显变化(p>0.05).结论 以上结果提示模拟失重后脊髓小脑中间区Ⅲ、Ⅳ小叶内神经环路的活动发生了改变,进一步证实了模拟失重可以导致肌肉本体感觉传人信息在高位中枢的分析整合过程中发生了变化.
objective to study the effects of 14 clays of simulated weightlessness on γ-aminobutyric acid (gaba) immunoreactivity in the cortex of lobule Ⅲ and Ⅳ of intermediate spinocerebellum and in the interposed eerebellar nucleus of rat. methods weightlessness was simulated by tail suspension of rats. by means of immunohistochemistry technique, changes of expression of gaba immunoreactivity in the cortex of lobule Ⅲ and Ⅳ of intermediate spinocerebellum and in the interposed cerebellar nucleus were detected both for the rats of 14-day tail suspension group (hu) (n=10)and control group (con) (n=10). results the results showed that gaba immunoreactive neurons were found in three layers (molecular layer, purkinje layer and granular layer) in the cortex of lobule Ⅲ and Ⅳ of intermediate spinocerebellum and interposed cerebellar nucleus in con and hu. however, after 14 days of tail-suspension, the number of gaba immunoreactivitive neurons in molecular layer was obviously decreased from 23.00±4.36 to 17.00± 3.25 comparing with that of con (t=3.488, p<0. 05). while the number of gaba immunoreactive neurons in the purkinje layer (7.42±1.82), granular layer (0.10±0.01) and interposed cerebellar nucleus (37.00±10.25) were not statistically different from those of the rats in con (7.18±2.31, 0.10±0.01 and 34.00±9.64, respectively) (p>0.05). conclusions our findings suggest that simulated weightlessness induces an obvious change in the activation of neuronal circuit in the lobule Ⅲ and Ⅳ of intermediate spinocerebellum and confirm further that the simulated weightlessness may result in muscle spindle afferent altered during central progressing.
出处
《中华航空航天医学杂志》
CSCD
2009年第3期212-215,封4,共5页
Chinese Journal of Aerospace Medicine
