摘要
观察了含有不同钙离子浓度的细胞外液对大鼠离体单一比目鱼肌肌梭自发放电的影响。实验分为低钙改良任氏液组和高钙改良任氏液组,首先采用空气隔绝法记录大鼠离体单一比目鱼肌肌梭自发放电情况,然后分别以相应改良任氏液灌流肌梭,并记录其自发放电和氯化琥珀胆碱所致的诱发放电的情况。给予低钙改良任氏液灌流肌梭后,其自发放电频率增加(P<0.01),自发放电的周期间隔缩短(P<0.01),给予氯化琥珀胆碱后诱发放电未见明显增加;给予高钙改良任氏液灌流肌梭后,其自发放电频率减少(P<0.01),自发放电的周期间隔延长(P<0.05),给予氯化琥珀胆碱后诱发放电未见明显增加。提示含有较低钙离子浓度的细胞外液可增加大鼠离体单一比目鱼肌肌梭自发放电,含有较高钙离子浓度的细胞外液可抑制该肌梭自发放电。为大鼠肌梭生理的研究及钙离子与失重性肌萎缩的实验研究提供了有意义的资料。
The impulse activities of muscle spindles isolated from the rat soleus muscle were investigated under varying concentrations of external calcium. The experiments were divided into low calcium modified Ringer＇s solution group and high calcium modified Ringer＇s solution group,using air gap technique to record the spontaneous discharge activities of isolated muscle spindle. And then with administrations of the corresponding modified Ringer＇s fluid,the changes of the spontaneous discharges and evoked discharges by Succinylcholine chloride were recorded. After administration of Modified Ringer＇s solution with low calcium,the spontaneous discharges of single muscle spindle were significantly increased（P〈0. 01）,the interval of spontaneous discharge was shortened（P〈0. 01）. After administration of Succinylcholine chloride,evoked discharges were not significantly increased. After administration of Modified Ringer＇s solution with high calcium,the spontaneous discharges of single muscle spindle were significantly decreased（P〈0. 01）,the interval of spontaneous discharge was prolonged（P〈0. 05）. After administration of Succinylcholine chloride,evoked discharges were not significantly increased. The extracellular fluid with a low concentration of calcium ions can increase the spontaneous discharges of single muscle spindle of rats＇ soleus muscle,and the extracellular fluid containing high calcium concentration can inhibit the spontaneous discharges of single muscle spindle of rats＇ soleus muscle.
作者
余蕾
赵雪红
樊小力
曹健
YU Lei;ZHAO Xue-hong;FAN Xiao-li;CAO Jian(Xi＇an Medical University, Xi＇an 710021;Hubei University of Arts and Science, Xiangyang 441053;Medical College, Xi＇an Jiaotong University, Xi＇an 710016, China)
出处
《生物学杂志》
CAS
CSCD
北大核心
2018年第2期60-62,76,共4页
Journal of Biology
基金
国家自然科学基金青年基金项目(31300981)
陕西省教育厅专项科研基金(2015JK1616)
关键词
钙离子
单一肌梭
自发放电
大鼠
Ca^2＋
single muscle spindle
spontaneous discharge
rats
