摘要
目的观察失重和噪声复合因素对大鼠胸腺细胞周期和细胞构成的影响,探讨航天条件下机体免疫功能降低的可能机制。方法大鼠给予模拟失重和/或噪声,分别于实验的第3、7、14天称大鼠体质量后处死,称胸腺质量,制备胸腺细胞悬液,采用流式细胞术测定胸腺细胞周期和胸腺细胞构成。结果与对照组相比,模拟失重和噪声复合组和模拟失重大鼠,体质量降低,噪声组大鼠体质量升高或无改变;复合组、模拟失重和噪声组大鼠均出现胸腺萎缩,G0/G1期细胞比例升高,S期和G2/M期细胞比例降低,胸腺细胞中CD4-CD8-、CD4+CD8-和CD4-CD8+T细胞比例升高,CD4+CD8+T细胞降低,但三组大鼠出现的时间不同。结论模拟失重和噪声对胸腺均具有显著的影响,但影响的程度不同,且两者具有叠加作用,可能是造成T细胞功能降低的原因之一。
Objective To observe the combined effects of simulated weightlessness and noise on the cell cycles of thymocytes and cell compositions of thymus in rats and to explore the possible mechanism of immune function depression in space flight. Methods SD rats were stimulated by simulated weightlessness and / or noise. On the 3rd,7th and 14 th day,the rats were weighed and then killed. The thymuses were taken,weighed and cell suspensions were made. Cell cycles and compositions in thymocytes were analyzed by flow cytometry. Results Compared with control group,the weights of rats were reduced in combined group and simulated weightlessness group,but the weights of rats increased or did not change in noise group. Rats in the three groups showed thymus atrophy. The ratio of cells increased in G0 / G1 phase and decreased in S and G2 / M phases. The ratios of CD4-CD8-,CD4＋CD8-and CD4-CD8＋T lymphocytes increased and CD4＋CD8＋T lymphocytes decreased. However,these changes occurred at different time points in different groups. Conclusion Simulated weightlessness and noise have significant effects on thymus,but the severity are different. The combined factors have superimposed effects. Maybe this is one of the reasons for depressed functions of T lymphocytes in space flight.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2016年第3期304-307,312,共5页
Chinese Journal of Cellular and Molecular Immunology
基金
军队十二五重大课题(AWS11J003)
关键词
模拟失重
噪声
胸腺
细胞周期
胸腺细胞构成
simulated weightlessness
noise
thymus
cell cycle
thymocyte composition
