摘要
目的研究痰热清注射液对Lewis肺癌化疗模型小鼠免疫功能的影响,探讨该药对肺癌小鼠的免疫调节作用。方法构建小鼠Lewis肺癌模型,分为模型对照组、单独痰热清治疗组、单独化疗组、联合痰热清化疗组,另设正常对照组。每组8只。取各组小鼠外周血后,引颈处死小鼠,取瘤组织、股骨、脾脏和胸腺用于后续实验。观察各组小鼠瘤组织质量、体积,流式细胞术检测各组瘤细胞凋亡水平及瘤组织CD3+T细胞、CD3-NK1.1+细胞浸润情况,HE染色观察胸腺组织结构,计算胸腺指数,并检测外周血淋巴细胞计数及股骨有核细胞总数、MTT法检测脾细胞毒性T细胞(CTL)杀伤活性。反转录PCR技术检测小鼠瘤组织表达γ干扰素(IFN-γ)、肿瘤坏死因子α(TNF-α)mRNA的水平。结果化疗后,联合痰热清化疗组小鼠胸腺指数、外周血淋巴细胞计数及股骨细胞总数均明显高于单独化疗组。化疗后,联合痰热清组小鼠瘤组织CD3+T细胞及CD3-NK1.1+细胞浸润程度均明显强于单独化疗组。化疗后,联合痰热清组小鼠瘤组织表达IFN-γ及TNF-αmRNA水平明显高于单独化疗组,脾CTL杀伤活性明显高于单独化疗组。结论痰热清注射液对肺癌化疗造成的机体的免疫功能损伤有明显保护作用,并对机体抗肿瘤免疫力有明显的促进作用。
Objective To investigate the effect of Tanreqing injection on immune function of mice with Lewis lung carcinoma treated by chemotherapy and discuss the immunoregulatory function of this herb. Methods All mouse models with Lewis lung carcinoma were randomly divided to four groups： model control group, Tanreqing injection group, chemotherapy group and chemotherapy combined with Tanreqing injection group （8 rats in each group）. Other 8 normal mice served as a normal control group. After treatment, peripheral blood was collected from the mice of all groups before they were sacrificed. Tumor tissue, femur, thymus and spleen were obtained to perform the following experiments. Tumor mass and volume were first observed. The apoptosis levels of tumor cells and the ratios of CD3^＋ T lymphocyte and CD3^- NK1. 1^＋ cells in the infiltrating lymphocytes in the tumor tissues were analyzed by flow cytometry. Thymus indexes （TI） were counted, and the structure of thymus was observed using HE staining. Cytotoxicity of spleen cytotoxic T cells （CTL） were investigated by MTT assay. The number of lymphocytes in the periphery blood and the nucleated cells in femur were also detected, and the expression levels of interferon-γ（IFN-γ） and tumor necrosis factor-α （TNF-α） mRNA in the tumor tissues were studied by reverse transcription PCFI. Results After chemotherapy, TI, the number of the nucleated cells in femur and lymphocytes in peripheral blood of chemotherapy combined with Tanreqing injection group were obviously higher than those in the chemotherapy group. The ratios of CD3 ^＋ T lymphocytes and CD3^- NK1. 1 ^＋ cells in the infiltrating lymphocytes in the tumor tissues of chemotherapy combined with Tanreqing injection group were also significantly higher than those in the chemotherapy group, Besides, compared with chemotherapy group, cytotoxicity of CTL in chemotherapy combined with Tanreqing injection group was improved notably. Meanwhile, the expression levels of IFN-γ and TNF-α mRNA in tumor tis
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2015年第3期297-301,共5页
Chinese Journal of Cellular and Molecular Immunology
基金
河北省中医药管理局指令性课题(2013018)
关键词
肺癌
痰热清注射液
化疗
免疫功能
lung carcinoma
tanreqing injection
chemotherapy
immune function
