摘要
目的观察黄芪多糖(APS)及联合顺铂(DDP)对Lewis肺癌(LLC)移植瘤小鼠凋亡蛋白细胞色素C(CytC)和高温必需丝氨酸蛋白酶A2(Omi/HtrA2)表达的影响。方法 90只C57BL/6J小鼠,随机分为正常组、模型组、(50、100、200)μg/mL APS处理组、6 mg/kg DDP处理组、3 mg/kg DDP联合(50、100、200)μg/mL APS处理组,每组10只。除正常组外,其余80只均接种1×107LLC细胞0.2 mL于右前肢腋窝皮下,建立荷瘤小鼠模型。造模次日起,治疗组的小鼠给予腹腔注射0.3 mL药物。每周注射1次DDP,其余药物每天1次,正常组和模型组注射等体积生理盐水,连续20 d,于第21天处死。采用HE染色观察肿瘤组织病变情况;免疫组织化学染色和图像分析法检测移植瘤细胞中的Cyt C及Omi/HtrA2的表达和定位。结果 (100、200)μg/mL APS处理及3 mg/kg DDP联合(100、200)μg/mL APS的荷瘤小鼠肿瘤质量减轻。与模型组小鼠比较,200μg/mL APS联合3 mg/kg DDP小鼠的肿瘤细胞坏死最为明显,各处理组的肿瘤组织中Cyt C、Omi/Htr A2蛋白水平均增加,200μg/mL APS联合3 mg/kg DDP小鼠的肿瘤组织增加最明显。结论APS及联合DDP能抑制小鼠Lewis肺癌细胞的生长,可能与肿瘤细胞Cyt C、Omi/Htr A2表达增加有关。
Objective To observe the effect of Astragalus polysaccharides （APS） combined with cisplatin （DDP） on the expressions of cytochrome C （CytC） and high temperature required sedne protease A2 （Omi/HtrA2） in the mice with Lewis lung carcinoma （LLC） transplantated tumors. Methods Ninty C57BL/6J mice were randomly divided into normal control group, model group, and （50, 100,200） μg/mL APS groups, 6 mg/kg DDP group, 3 mg/kg DDP combined with （50, 100, 200）μg/mL APS groups. Each group included 10 mice. Except the mice in the normal group, the rest mice were inoculated subcutaneously with LLC cells （ 1× 10^7 mL） at the right fore axillary fossa to establish tumor-bearing mouse models. In the second day of building models, the mice in the treatment group were given intraperitoneal injection of 0. 3 mL of the drug. DDP was given once a week, and the other drugs once a day. The mice in the normal group and the model group were administrated the same amount of saline injection for continuous 20 days. All mice were killed at the 21st day. The pathological changes of tumor tissues were observed by HE staining. The expressions and location of CytC and Omi/HtrA2 proteins in the transplanted tumor tissues were detected by immunohistochemical staining and image analysis. Results The mass of tumor decreased in the mice of （100,200） μg/mL APS group and 3 mg/kg DDP combined with （100, 200） μg/mL APS group. Compared with the model group, the necrosis of tumor tissues in 200 μg/mL APS combined with 3 mg/kg DDP group was the most obvious. The expressions of CytC and Omi/HtrA2 increased in the treatment groups, and the increase was the most remarkable in 200 μg/mL APS combined with 3 mg/kg DDP group. Conclusion APS and APS combined with DDP can restrain the growth of Lewis Lung cancer in C57BL/6J mice, which may be related to the increased expressions of CytC and OmVHtrA2.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2017年第4期503-507,共5页
Chinese Journal of Cellular and Molecular Immunology
基金
甘肃省高校基本科研业务项目(2014)
甘肃省科技厅计划项目(148RJZA060)
兰州市科技局计划项目(2014-1-23)
