摘要
目的探讨生后锰接触对小鼠中脑被盖相关核区发育的毒性影响。方法采用口服MnCl2水溶液途径给仔鼠染锰,应用旷场迷宫检测仔鼠自发活动兴奋性改变、Morris水迷宫试验检测仔鼠方位学习记忆的改变;应用荧光分光度计法检测中脑单胺类递质含量变化;应用细胞免疫化学(ABC法)与图像分析相结合技术检测中脑被盖部相关核区酪氨酸羟化酶(TH)免疫反应阳性神经元的反应强度和TH阳性产物平均相对光密度的变化。结果随染锰剂量的增大,仔鼠自发活动次数明显增加,到达水迷宫平台的潜伏期显著延长,中脑脑段的单胺类神经递质多巴胺(DA)、去甲基肾上腺素(NE)和5鄄羟色胺(5鄄HT)含量水平明显下降,中脑被盖相关核区TH阳性神经元免疫反应强度显著减弱、TH阳性产物平均相对光密度明显降低。结论生后锰接触对仔鼠中脑相关核区的发育造成明显毒性损害,其损害程度随染锰剂量的加大而加重。
Objective To explore the poisonous effects of manganese exposure on midbrain development in mice. Methods Spontaneous action was examined by open field maze. average latency to reach the plateform below surface of water was mecisured by Morris water maze. Monoamine contents of midbrain were measured by fluorescent analysis. Tyrosine hydroxylase(TH)immunoreactivity and its proportional densities were detected by cytoimmunochemistry combined with imaging analysis. Results Significant increased spontaneous action and delay of latency to the plateform below water, as well as decreased monoamine contents in midbrain were observed with Mn-exposured increased. The numbers of TH-positive neurons and its processes, as well as its average proportional desities in the related nuclei(or areas) of midbrain were significantly decreased with Mn-exposured increased. Conclusion The toxicity of manganese expoure is obvious on the postnatal development in mice midbrain.
出处
《解剖学研究》
CAS
2005年第1期7-9,F004,共4页
Anatomy Research
基金
国家自然科学基金(No.39560072)
关键词
中脑
锰接触
仔鼠
小鼠
改变
自发活动
毒性
发育
产物
兴奋性
Manganese expoure
Postnatal development
Toxicity
Midbrain
Tyrosine hydroxylase
Mice
