摘要
目的 观察艾灸对慢性应激状态下大鼠下丘脑 垂体 肾上腺轴功能的保护作用。方法 SD雄性大鼠 39只 ,随机分为正常组、模型组、艾灸组。应用分养和长期不可预见性的中等强度刺激造成应激失调模型 ,观察各组大鼠体重、蔗糖偏嗜度、旷场实验等行为学改变。采用放射免疫分析法观察艾灸“大椎”穴对模型大鼠血浆皮质醇、促肾上腺皮质激素的影响。并用免疫组织化学和计算机图象分析法比较各组动物下丘脑室旁核精氨酸加压素阳性神经元的数量。采用HE染色、尼氏体染色的方法观察海马神经元形态的改变。结果 慢性应激可致大鼠体重增加克数、蔗糖偏嗜度、旷场实验中活动次数较正常组显著减少。血浆皮质醇、促肾上腺皮质激素含量及下丘脑室旁核精氨酸加压素阳性神经元的数量明显高于正常组。海马神经元形态明显受损 ,形态以空泡为主。艾灸对以上症状和指标有明显改善作用。结论 慢性应激导致大鼠HPA轴功能明显亢进 ,艾灸“大椎”
Objective To observe the antagonism of moxibustion on stress. Method Forty male Sprague-Dawley rats were randomly divided into control group, model group and Moxibustion group. All rats except the control group singly housed and exposed chronically 21 days to a variety of mild unpredictable stressors, and the rats of Moxibustion group accepted Moxibustion treatment at the same time. The behavior of rats was detected by sucrose preference test and open-field test. Observe the levels of adrenocorticotropic hormone(ACTH) and cortisol(CORT) in plasma in different groups. Use immunocytochemistry method and computer to quantitatively analyse the expression levels of arginine vasopressin(AVP) in paraventricular nucleus(PVN) of the hypothalamus. With method of histochemistry and immuno-histochemistry ,we observe the structure of hippocampal neurons and measure the BDNF infected neurons. Results Compared with control group, the increased weight、 the sucrose preference and activity of rats in open-field test in model group decreased significantly. Results also showed that the concentration of plasma ACTH and CORT, the number of AVP infected neurons in PVN increased significantly in stressed-model rats compared to controls. The number of hipoccampal neurons decreased with cell structure destroyed. Immuno-histochemistry results showed that the number of infected neurons of model animals was much less than that of normal animals. Moxibustion can improve behavioral disorder. Levels of ACTH, CORT and AVP markedly dropped in Moxibustion group. And that moxbustion can protect the hippocampal neurons in the depressed animals. Conclusion Moxbustion has antagonism on stress.
出处
《中国行为医学科学》
CSCD
2002年第5期495-497,共3页
Chinese Journal of Behavioral Medical Science
