摘要
目的:通过观察电针“大椎”“命门”穴后下丘脑CRH、血浆ACTH、血清Cort、TNF-α含量的关系,分析电针不同穴位对应激相关因子的影响。方法:以佐剂性关节炎大鼠(AA)作为研究对象,随机分正常组、模型组、电针“大椎”组、电针“命门”组。观察电针对关节炎症局部及下丘脑CRH、血浆ACTH、血清Cort、TNF-α含量的影响。结果:“大椎”组、“命门”组经治疗右后足足爪肿胀率与模型对照组比较极明显降低(P<0.01),“大椎”组下丘脑CRH含量、血清Cort、TNF-α含量,“命门”组血清Cort含量与模型对照组比较明显降低(P<0.05)。结论:电针具有一定的抗炎作用,并且这种作用可能是通过对CRH、Cort等应激相关因子的调节,减轻疾病应激程度而实现的。
Objective： To study the effect of electroacupuncture （EA） of ＂Dazhui＂ （GV 14） and ＂Mingmen＂ （GV 4） on hypothalamic corticotrophin releasing hormone （CRH）, plasma adrenocorticotropic hormone （ACTH）, serum cortisol （Cort） and tumor necrosis factor （TNF）-α contents in adjuvant arthritis rats. Methods： Thirty-six male Wistar rats were evenly randomized into normal control, model, EA-GV14 and EA-GV4 groups. Arthritis model was established by subcutaneous injection of Freund＇s complete adjuvant （FCA, 0.1 mL） into the right hind-paw. EA （15 Hz, continuous pulses, 1 mA） was applied to ＂Dazhui＂（GV 14） and ＂Mingrnen＂ （GV 4） for 20 min, once every other day and continuously for 8 times. After the rats were killed, hypothalam- ic tissue and blood sample were taken for detecting hypothalamic CRH, plasma ACTH, serum Cort and TNF-α contents with radioimmunoassay. Results： Compared with normal control group, the swelling rate of the right hindpaw 24 h after injection of FCA and 16 days after EA increased significantly （P〈 0.01 ）; while in comparison with model group, the swelling rates of EA-GV14 and EA-GV4 groups were markedly lower （P〈0.01）. Compared with normal control group, hypothalamic CRH, plasma ACTH, serLrn Cort and TNF-α contents of model group increased significantly （ P 〈 0.05） ; while in comparison with model group, hypothalamic CRH serum Cort and TNF-α contents in EA-GV14 group, and serum Cort level in EA-GV4 group decreased significantly （ P〈 0.05）. No significant differences were found between EA-GV14 and EA-GV4 groups （ P 〉 0.05） in these 4 indexes. Conclusion： EA has a marked anti-inflammatory effect in adjuvant arthritis rats and can relieve arthritis stress induced increase of hypothalamic CRH, plasma ACTH, serum Cort and TNF-α levels.
出处
《针刺研究》
CAS
CSCD
2006年第1期8-11,共4页
Acupuncture Research
基金
国家自然科学基金课题(90209026)
教育部优秀青年骨干教师科研资金资助
关键词
佐剂性关节炎
电针“大椎”“命门”
应激相关因子
Adjuvant arthritis Electroacupuncture ＂Dazhui＂ （GV 14） ＂Mingrnen＂ （GV 4） Stres.^-related factors
