摘要
目的 :制定复方延胡索片中延胡索乙素的含量测定方法。方法 :双波长薄层扫描法 ,以苯 石油醚 甲醇 三氯甲烷 (3∶3∶0 .5∶10 )为展开剂 ,样品波长 3 40nm ,参比波长 3 65nm。结果 :回收率平均 98.2 % (RSD =1.88% )。标准曲线r =0 .9996,线性范围 0 .2～ 1.4μg。 结论 :该法简便、准确 ,可作为复方延胡索片的质控方法。
Objective:To develop a method for the determination of dl tetrahydropalmatine in compound rhizoma corydalis tablets. Method:Dl tetrahydropalmatine was assayed by dual wavelength TLC Scanning method with 340 nm and 365 nm as the sample and reference wavelength respectively. The mobile phase was benzene petroleum ether (90～120℃) methanol chloroform (3∶3∶0.5∶10). Results: The average recovery was 98.2% ( RSD =1.88%, n =5) with good linear relationship (r=0.999 6). The linear range was from 0.2 to 1.4 μg. Conclusion:The method was simple and accurate and can be used for the quality control of compound rhizoma corydalis tablets.
出处
《医药导报》
CAS
2002年第11期733-734,共2页
Herald of Medicine
关键词
薄层扫描法
复方延胡索片
延胡索乙素
含量测定
Dl tetrahydropalmatine
Rhizoma corydalis tablets, compound
TLC Scanning
Content determination
